P5208
Platelet-Derived Endothelial Cell Growth Factor human
recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture
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Synonym(s):
PD-ECGF
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biological source
human
Quality Level
recombinant
expressed in baculovirus infected Sf21 cells
Assay
≥97% (SDS-PAGE)
form
lyophilized powder
potency
5-30 ng/mL EC50
mol wt
49 kDa
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
<1 EU/mg
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... PDGFA(100126841) , TYMP(1890)
General description
Platelet-Derived Endothelial Cell Growth Factor (PD-ECGF) is an endothelial cell mitogen which was originally purified from human platelets. In contrast with the endothelial mitogens of the fibroblast growth factor (FGF) family, PD-ECGF is an endothelial cell growth factor initially purified from human platelets, which does not bind to heparin and does not stimulate the proliferation of fibroblasts. This is in contrast with the effect of PDGF, which stimulates the growth of human foreskin fibroblasts, but is inactive on endothelial cells. PD-ECGF will stimulate endothelial cells in vitro and in vivo. PD-ECGF is chemotactic for bovine aortic endothelial cells. PD-ECGF does not induce smooth muscle cell migration. PD-ECGF is involved in angiogenesis and has potent angiogenic activity both in the developing vascular system of the chick chorioallantoic membrane and in vascularization of tumors in nude mice. PD-ECGF has a pI of 4.0-4.8 and a molecular weight of 49 kDa.
The biological activity of recombinant, human PD-ECGF is measured in a cell proliferation assay using human umbilical vein endothelial cells. The EC50 is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay
The biological activity of recombinant, human PD-ECGF is measured in a cell proliferation assay using human umbilical vein endothelial cells. The EC50 is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay
Application
PD-ECGF is involved in angiogenesis and has potent angiogenic activity both in the developing vascular system of the chick chorioallantoic membrane and in vascularization of tumors in nude mice.
Biochem/physiol Actions
Platelet-derived endothelial cell growth factor is a 45 kDa endothelial cell mitogen of pI 4.0-4.8 originally purified from human platelets. PD-ECGF is chemotactic for bovine aortic endothelial cells, however it does not induce smooth muscle cell migration. PD-ECGF is involved in angiogenesis and has potent angiogenic activity both in the developing vascular system of the chick chorioalantoic membrane and vascularization of tumor cells in nude mice. PD-ECGF is highly expressed in some tumors.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.
Analysis Note
The proliferative activity of human PD-ECGF is measured in culture by using human umbilical vein endothelial cells.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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H Aikawa et al.
Anticancer research, 19(5B), 4305-4309 (2000-01-11)
In order to elucidate the roles of tumor angiogenesis in lung carcinogenesis, the expressions of several angiogenic factors in lung carcinoma tissues were examined. Tissue specimens from 112 cases of resected non-small cell lung cancer (NSCLC) were studied. The expressions
Jue Li et al.
Bioengineered, 13(3), 4844-4856 (2022-02-15)
The association between spinal cord injury (SCI) and bladder symptoms has been intensively described. Human umbilical cord mesenchymal stem cell (hUC-MSC) treatment is beneficial to the recovery of bladder function after SCI, but its mechanism is unclear. We established an
L Griffiths et al.
British journal of cancer, 76(6), 689-693 (1997-01-01)
Angiogenesis plays an important role in the growth and metastasis of solid tumours. Platelet-derived endothelial cell growth factor (PD-ECGF) is known to be chemotactic for endothelial cells in vitro and angiogenic in vivo. It is also known as gliostatin, a
C-S Bao et al.
European review for medical and pharmacological sciences, 22(19), 6436-6447 (2018-10-20)
This research aimed to investigate the therapeutic effects of transplanted human umbilical cord mesenchymal stem cells (hUCMSCs) on spinal cord injury in mice and to explore its molecular mechanism. Spinal cord injury model in C57BL/6J mice was established. On the
Sinsuk Han et al.
Autophagy, 17(12), 4182-4201 (2021-03-25)
Mitochondria are the main cellular energy powerhouses and supply most of the energy in the form of ATP to fuel essential neuronal functions through oxidative phosphorylation (OXPHOS). In Alzheimer disease (AD), metabolic and mitochondrial disruptions are an early feature preceding
Articles
Platelet-Derived Growth Factor is the principal mitogen found in mammalian serum and is released from platelets during clot formation.
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