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P3326

Sigma-Aldrich

Platelet-Derived Growth Factor-AB human

PDGF-AB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):

PDGF-AB

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About This Item

MDL number:
MFCD00213024
UNSPSC Code:
12352202
NACRES:
NA.77

biological source

human

Quality Level

200

recombinant

expressed in E. coli

Assay

≥97% (SDS-PAGE and N-terminal analysis)

form

lyophilized powder

potency

10-100 ng/mL ED50/EC50

quality

endotoxin tested

mol wt

27-31 kDa

packaging

pkg of 10 μg
pkg of 50 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg

UniProt accession no.

P04085, P01127

storage temp.

−20°C

Gene Information

human ... PDGFA(5154) , PDGFB(5155)

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General description

Platelet-Derived Growth Factor (PDGF) is a cationic polypeptide and a constituent of α-granules. PDGF is composed of chain A and B linked together by a disulphide bridge. PDGF heterodimers occurs as PDGF-AB and homodimers exists as FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD). The PDGFB gene is localized to pericytes in the brain and is mapped to human chromosome 7p22.3.

Application

Platelet-Derived Growth Factor-AB human has been used in luciferase reporter assay.

Biochem/physiol Actions

Cellular actions of platelet-derived growth factor (PDGF) include chemoattraction and activation of neutrophils, monocytes, and fibroblasts. PDGF appears to play a crucial role in the cellular response to tissue injury and is critically involved in the pathological progression of atherosclerosis.
PDGF stimulates fibroblast proliferation and extracellular matrix production in order to facilitate tissue repair. PDGF-AB isomer is found to be associated with angiogenesis and proliferation. Loss of PDGFB gene function induces microvascular calcification due to age-dependent phenotypical changes.

Other Notes

Heterodimer of the A and B chains of PDGF disulfide linked.

Physical form

Lyophilized from a 0.2 μm filtered solution in 40% acetonitrile and 0.1% trifluoroacetic acid.

Analysis Note

The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Increased levels of platelet-derived growth factor in vitreous fluid of patients with proliferative diabetic retinopathy.
Freyberger H, et al.
Experimental and Clinical Endocrinology & Diabetes, 108.02, 106-109 (2000)
Injury induces in vivo expression of platelet-derived growth factor (PDGF) and PDGF receptor mRNAs in skin epithelial cells and PDGF mRNA in connective tissue fibroblasts.
Antoniades HN, et al.
Proceedings of the National Academy of Sciences of the USA, 88.2 , 565-569 (1991)
Intraglomerular expressions of IL-1a and platelet-derived growth factor (PDGF-B) mRNA in experimental immune complex-mediated glomerulonephritis.
Akai Y, et al.
Clinical and Experimental Immunology, 95.1, 29-34 (1994)
Makoto Hiromura et al.
The Journal of biological chemistry, 281(38), 27753-27764 (2006-07-13)
The serine/threonine kinase, Akt (protein kinase B) plays a central role in the regulation of intracellular cell survival. Recently, we demonstrated that the proto-oncogene TCL1, overexpressed in human T-cell prolymphocytic leukemia, is an Akt kinase co-activator. Tightly restricted TCL1 gene
Identification of nerve growth factor-responsive element of the TCL1 promoter as a novel negative regulatory element.
Hiromura M, et al.
The Journal of Biological Chemistry, 281.38, 27753-27764 (2006)
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Articles

Platelet Derived Growth Factors (PDGF)

Platelet-Derived Growth Factor is the principal mitogen found in mammalian serum and is released from platelets during clot formation.

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