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P3201

Sigma-Aldrich

Platelet-Derived Growth Factor-BB human

PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):

PDGF-BB

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About This Item

MDL number:
MFCD00213023
UNSPSC Code:
12352202
NACRES:
NA.77

biological source

human

Quality Level

200

recombinant

expressed in E. coli

Assay

≥98% (HPLC and SDS-PAGE)

form

lyophilized powder

potency

1.0-3.0 ng/mL EC50

quality

endotoxin tested

mol wt

~25 kDa

packaging

pkg of 10 μg
pkg of 50 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg

UniProt accession no.

P01127

storage temp.

−20°C

Gene Information

human ... PDGFB(5155)

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General description

Platelet-derived growth factor (PDGF) is expressed as homo and heterodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD. The PDGF gene is highly conserved mapped to human chromosome 7p22.3. The active form of Pdgf exists as disulfide-linked dimers. It consists of cystine-knot-fold growth factor domain, which is highly conserved, except for the N-terminal prodomain, which shows variation in length among the isoforms.

Application

Platelet-Derived Growth Factor-BB human has been used :
  • As a growth supplement in proximal tubule and glomerular explant cell culture
  • In three-dimensional scaffold preparation
  • To study the effect of brivanib on growth factor signaling pathways
  • In transwell migration assay

Biochem/physiol Actions

Platelet-derived growth factor (PDGF) elicits multifunctional actions with a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells, and chondrocytes. PDGF significantly activates chemotaxis of neutrophils, monocytes, and fibroblasts. It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins, and modulates LDL (low density lipoprotein) receptor binding.
PDGF stimulates fibroblast proliferation and extracellular matrix production in order to facilitate tissue repair. PDGF-BB isomer is found to be critical in angiogenesis and is highly expressed in tumor, mediating oncogenic pericyte recruitment. PDGF-BB is an important regulator of bone homeostasis, repair and regeneration.

Other Notes

Dimer of the B chain of human PDGF.

Physical form

Lyophilized from a 0.2 μm filtered solution in 30 mM acetic acid.

Analysis Note

The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Clinical Findings Associated with a De Novo Partial Trisomy 10p11. 22p15. 3 and Monosomy 7p22. 3 Detected by Chromosomal Microarray Analysis.
Kohannim O,et al.
Case Reports in Genetics, 2011 , 1-6 (2011)
Yasutoshi Takashima et al.
American journal of physiology. Renal physiology, 310(5), F395-F408 (2016-01-15)
Glomerular integrity and functions are maintained by growth factor signaling. Heparan sulfate, the major component of glomerular extracellular matrixes, modulates growth factor signaling, but its roles in glomerular homeostasis are unknown. We investigated the roles of heparan sulfate 6-O-endosulfatases, sulfatase
Matrix stiffness regulates migration of human lung fibroblasts.
Asano1 S, et al.
Physiological Reports, 5.9, 1-11 (2017)
Brivanib attenuates hepatic fibrosis in vivo and stellate cell activation in vitro by inhibition of FGF, VEGF and PDGF signaling.
Nakamura I, et al.
PLoS ONE, 9.4 (2014)
Injury induces in vivo expression of platelet-derived growth factor (PDGF) and PDGF receptor mRNAs in skin epithelial cells and PDGF mRNA in connective tissue fibroblasts
Antoniades HN
Proceedings of the National Academy of Sciences of the USA, 88.2, 565-569 (1991)
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