P0520
Phosphodiesterase 3′,5′-Cyclic Nucleotide Activator-deficient from bovine heart
lyophilized powder (contains imidazole buffer salts and magnesium sulfate), >0.1 units/mg protein (with added activator), PI fraction of Ho, H.C., et al., Biochim. Biophys. Acta, 429, 461 (1976).
Synonym(s):
3′,5′-Cyclic-nucleotide 5′-nucleotidohydrolase from bovine heart, PDE
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About This Item
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biological source
bovine heart
form
lyophilized powder (contains imidazole buffer salts and magnesium sulfate)
specific activity
>0.1 units/mg protein (with added activator)
mol wt
~60 kDa
composition
Protein, ~75%
UniProt accession no.
foreign activity
5′-ATPase, alkaline phosphatase, and 2′:3′-cyclic nucleotide phosphodiesterase <0.005 units/mg protein
5′-nucleotidase <0.01 units/mg protein
inorganic pyrophosphatase <0.5 units/mg protein
storage temp.
−20°C
Gene Information
cow ... PDE1A(281969)
Application
Cyclic nucleotide phosphodiesterase has been investigated for its mechanism of activation as a function of calmodulin and Ca2+. It has also been used in a study to show that the binding of trifluoperazine to the activator of cyclic nucleotide phosphodiesterase increases the activity by over 10-fold.
For use in calmodulin (P 0270) assay. Activity is reduced to <50% without calmodulin present.
Biochem/physiol Actions
Hydrolyzes the 3′,5′-phosphodiester bond in cyclic nucleotide monophosphates, such as cAMP and cGMP, to the corresponding nucleotide 5′-monophosphate.
The herbicide Atrazine was shown inhibit phosphodiesterase 3′,5′-Cyclic Nucleotide Activator-deficient from bovine heart.
Packaging
Package size based on activated units
Unit Definition
One unit will hydrolyze 1.0 μmole of 3′:5′,-cyclic-AMP to 5′-AMP per min at pH 7.5 at 30 °C.
Preparation Note
Crude preparation which has been stripped of the protein activator, calmodulin.
Reconstitution
Reconstitute with 50% glycerol. The total activated units of enzyme will remain constant for at least 5 days when stored at −0 °C. However, the activator deficient activity may increase up to 200%. If stored at 4 °C, both the activated and activator deficient activity, may decrease approx. 30% in 24 hrs.
Analysis Note
Protein determined by Warburg-Christian unless specified otherwise.
inhibitor
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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N Seiler et al.
The international journal of biochemistry & cell biology, 30(3), 393-406 (1998-06-05)
N1-Dansylspermine and related sulfonamides of the natural polyamines are very potent blockers of NMDA-type glutamate receptors. They exhibit pharmacological properties which were not predicted from the constituents of the conjugates. Cytotoxicity and calmodulin antagonism of N1-dansylspermine were especially impressive. Calmodulin
P J Bekker et al.
Calcified tissue international, 51(4), 312-316 (1992-10-01)
Plasma membrane vesicles were prepared from chicken osteoclasts, and active calcium transport was demonstrated in a spectrofluorimetric assay using the fluorescent calcium concentration indicator, fura-2. Transport activity was inhibited by quercetin (10 microM), sodium vanadate (10 microM), and the anticalmodulin
Binding of trifluoperazine to the calcium-dependent activator of cyclic nucleotide phosphodiesterase.
R M Levin et al.
Molecular pharmacology, 13(4), 690-697 (1977-07-01)
Evidence that calmodulin may be involved in phytohaemagglutinin-stimulated lymphocyte division.
S Mac Neil et al.
Bioscience reports, 2(11), 891-897 (1982-11-01)
T Kaneko et al.
Cellular signalling, 7(5), 527-534 (1995-07-01)
Elevated intracellular cyclic AMP is associated with the inhibition of many inflammatory cellular responses. In this study, we examined the effect of cyclic AMP on eosinophil chemotaxis. Eosinophils were isolated from healthy human volunteers using an immunomagnetic method. Eosinophils were
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