P0134
Phosphodiesterase, 3′,5′-cyclic-nucleotide-specific from bovine brain
lyophilized powder, ~0.2 units/mg protein
Synonym(s):
3′,5′-Cyclic-nucleotide 5′-nucleotidohydrolase from bovine brain, PDE
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About This Item
CAS Number:
UNSPSC Code:
12352204
EC Number:
232-920-5
MDL number:
form
lyophilized powder
specific activity
~0.2 units/mg protein
mol wt
~60 kDa
composition
Protein, ~80%
UniProt accession no.
storage temp.
−20°C
Gene Information
cow ... PDE1A(281969)
Application
For assay of 3′,5′-cyclic nucleotides.
Biochem/physiol Actions
Hydrolyzes the 3′,5′-phosphodiester in cyclic nucleotide monophosphates, such as cAMP and cGMP, to the corresponding nucleotide 5′-monophosphate.
Physical form
Lyophilized preparation containing near saturation levels of both protein activator and Ca++, and buffer salts as imidazole and magnesium sulfate
Preparation Note
Prepared as described by Butcher, R.W. and Sutherland, E.W., J. Biol. Chem., 237, 1244 (1962).
Reconstitute with 50% glycerol. The total activated units of enzyme will remain constant for at least 5 days when stored at −0 °C. However, the activator deficient activity may increase up to 200%. Both the activated and activator deficient activity may decrease approx. 30% in 24 hrs. if stored at 4 °C.
Analysis Note
Contains inorganic pyrophosphatase and 5′-ATPase.
Substantially free of 5′-nucleotidase and 2′,3′-cyclic nucleotide phosphodiesterase.
Substantially free of 5′-nucleotidase and 2′,3′-cyclic nucleotide phosphodiesterase.
Protein determined by Warburg-Christian unless specified otherwise.
Other Notes
One unit will hydrolyze 1.0μmole of 3′,5′-cyclic-AMP to 5′-AMP per min at pH7.5 at 30°C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Ryan T Cameron et al.
Biochemical pharmacology, 85(9), 1297-1305 (2013-03-12)
PDE4 is one of eleven known cyclic nucleotide phosphodiesterase families and plays a pivotal role in mediating hydrolytic degradation of the important cyclic nucleotide second messenger, cyclic 3'5' adenosine monophosphate (cAMP). PDE4 inhibitors are known to have anti-inflammatory properties, but
The cyclic AMP pathway.
Paolo Sassone-Corsi
Cold Spring Harbor perspectives in biology, 4(12), doi:10-doi:10 (2012-12-05)
Chimed Jansen et al.
Journal of medicinal chemistry, 56(5), 2087-2096 (2013-02-16)
Trypanosoma brucei cyclic nucleotide phosphodiesterase B1 (TbrPDEB1) and TbrPDEB2 have recently been validated as new therapeutic targets for human African trypanosomiasis by both genetic and pharmacological means. In this study we report the crystal structure of the catalytic domain of
N B Matveeva et al.
Biofizika, 57(5), 832-839 (2012-11-10)
Possible involvement of autocrine factors into the control of motile behavior via a receptor-mediated mechanism was investigated in Physarum polycephalum plasmodium, a multinuclear amoeboid cell with the auto-oscillatory mode of motility. Cyclic adenosine monophosphate (cAMP) and extracellular cAMP-specific phosphodiesterase, its
Tamam El-Elimat et al.
Journal of natural products, 76(3), 382-387 (2013-01-11)
Three bioactive compounds were isolated from an organic extract of an ascomycete fungus of the order Chaetothyriales (MSX 47445) using bioactivity-directed fractionation as part of a search for anticancer leads from filamentous fungi. Of these, two were benzoquinones [betulinan A
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