P0134
Phosphodiesterase, 3′,5′-cyclic-nucleotide-specific from bovine brain
lyophilized powder, ~0.2 units/mg protein
Synonym(s):
3′,5′-Cyclic-nucleotide 5′-nucleotidohydrolase from bovine brain, PDE
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About This Item
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form
lyophilized powder
specific activity
~0.2 units/mg protein
mol wt
~60 kDa
composition
Protein, ~80%
UniProt accession no.
storage temp.
−20°C
Gene Information
cow ... PDE1A(281969)
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Application
For assay of 3′,5′-cyclic nucleotides.
Biochem/physiol Actions
Hydrolyzes the 3′,5′-phosphodiester in cyclic nucleotide monophosphates, such as cAMP and cGMP, to the corresponding nucleotide 5′-monophosphate.
Quality
Contains inorganic pyrophosphatase and 5′-ATPase.
Substantially free of 5′-nucleotidase and 2′,3′-cyclic nucleotide phosphodiesterase.
Substantially free of 5′-nucleotidase and 2′,3′-cyclic nucleotide phosphodiesterase.
Unit Definition
One unit will hydrolyze 1.0μmole of 3′,5′-cyclic-AMP to 5′-AMP per min at pH7.5 at 30°C.
Physical form
Lyophilized preparation containing near saturation levels of both protein activator and Ca++, and buffer salts as imidazole and magnesium sulfate
Preparation Note
Prepared as described by Butcher, R.W. and Sutherland, E.W., J. Biol. Chem., 237, 1244 (1962).
Reconstitution
Reconstitute with 50% glycerol. The total activated units of enzyme will remain constant for at least 5 days when stored at −0 °C. However, the activator deficient activity may increase up to 200%. Both the activated and activator deficient activity may decrease approx. 30% in 24 hrs. if stored at 4 °C.
Analysis Note
Protein determined by Warburg-Christian unless specified otherwise.
inhibitor
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Ryan T Cameron et al.
Biochemical pharmacology, 85(9), 1297-1305 (2013-03-12)
PDE4 is one of eleven known cyclic nucleotide phosphodiesterase families and plays a pivotal role in mediating hydrolytic degradation of the important cyclic nucleotide second messenger, cyclic 3'5' adenosine monophosphate (cAMP). PDE4 inhibitors are known to have anti-inflammatory properties, but
The cyclic AMP pathway.
Paolo Sassone-Corsi
Cold Spring Harbor perspectives in biology, 4(12), doi:10-doi:10 (2012-12-05)
Stephen M Kraynik et al.
Molecular pharmacology, 83(6), 1155-1165 (2013-03-16)
Brown adipose tissue (BAT) is a highly thermogenic organ that converts lipids and glucose into heat. Many of the metabolic and gene transcriptional hallmarks of BAT activation, namely increased lipolysis, uncoupling protein-1 (UCP1) mRNA, and glucose uptake, are regulated by
One single signaling pathway for so many different biological functions: lessons from the cyclic adenosine monophosphate/protein kinase A pathway-related diseases.
Guillaume Assié
The Journal of clinical endocrinology and metabolism, 97(12), 4355-4357 (2012-12-12)
Yang Li et al.
The Journal of biological chemistry, 288(16), 11358-11365 (2013-03-12)
Ras proteins and cAMP-dependent protein kinase (protein kinase A, PKA) are important components of a nutrient signaling pathway that mediates cellular responses to glucose in yeast. The molecular mechanisms that regulate Ras/PKA-mediated signaling remain to be fully understood. Here, we
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