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N9891

Sigma-Aldrich

Monoclonal Anti-Nebulin antibody produced in mouse

clone NB2, ascites fluid

Synonym(s):

Anti-AMC6, Anti-NEB177D, Anti-NEM2

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About This Item

MDL number:
MFCD00164706
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

NB2, monoclonal

mol wt

antigen 500 kDa

contains

15 mM sodium azide

species reactivity

vertebrates, chicken

technique(s)

indirect immunofluorescence: 1:400 using frozen sections of animal skeletal muscle
western blot: suitable

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

chicken ... NEB(101748402)

Related Categories

General description

Nebulin is a 700kDa protein which is expressed in skeletal muscles. It possesses 35 amino acid repeats and a C terminal serine-rich and SH3 domains.
Nebulin is also localized to cardiac muscle. Monoclonal Anti-Nebulin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Specificity

Localizes nebulin in skeletal muscle of a wide variety of species from cold-blooded vertebrates to man. Does not crossreact with smooth or cardiac muscle, non-muscle tissue and cultured cells. Localizes nebulin within the I band of skeletal muscle sections by indirect immunofluorescence.

Immunogen

chicken breast nebulin.

Application

Monoclonal Anti-Nebulin antibody produced in mouse has been used in:
  • immunohistochemistry
  • fluorescent immunostaining
  • western blotting

Biochem/physiol Actions

Nebulin associates with the Z-line and thin actin filament in a sarcomere. It may function as a regulator of sarcomere thin filament length. The protein also acts as a structural link between sarcomeres. Nebulin interacts with the capping protein CapZ and has a role in controlling the localization of actin filaments.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Nebulin interacts with CapZ and regulates thin filament architecture within the Z-disc.
Pappas CT
Molecular Biology of the Cell (2008)
Biallelic mutations in MYPN, encoding myopalladin, are associated with childhood-onset, slowly progressive nemaline myopathy
Miyatake S, et al.
American Journal of Human Genetics, 100(1), 169-178 (2017)
J Q Zhang et al.
Biophysical journal, 74(1), 349-359 (1998-02-04)
cDNA clones encoding mouse skeletal muscle nebulin were expressed in Escherichia coli as thioredoxin fusion proteins and purified in the presence of 6 M urea. These fragments, called 7a and 8c, contain 28 and 19 of the weakly repeating approximately
Nebulin interacts with CapZ and regulates thin filament architecture within the Z-disc
Pappas CT, et al.
Molecular Biology of the Cell, 19(5), 1837-1847 (2008)
Clinical phenotype, muscle MRI and muscle pathology of LGMD1F
Peterle E, et al.
Journal of Neurology, 260(8), 2033-2041 (2013)
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