N6009
Nerve Growth Factor-2.5S from murine submaxillary gland
NGF-2.5S, lyophilized powder, suitable for cell culture
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Synonym(s):
NGF-2.5S
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biological source
mouse
Quality Level
form
lyophilized powder
potency
0.1-30 ng/mL EC50 (using PC-12 cells)
mol wt
26.5 kDa
packaging
pkg of 4X25 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
mouse ... Ngf(18049)
Related Categories
General description
Nerve growth factor (NGF) is produced by different cell types: structural, accessory, and immune cells. It belongs to the neurotrophin family of trophic factors.
Application
Nerve Growth Factor-2.5S from murine submaxillary gland has been used:
- in Ham′s F-12 medium for performing growth cone collapse assay using dorsal root ganglions (DRGs)
- as a media supplement in the “process compartment” for microfluidic chamber studies
- to treat mid-luteal cells to study its effect on choline acetyltransferase (CHAT) expression
Biochem/physiol Actions
Nerve growth factor (NGF) is neurotrophic and maintains sympathetic and sensory neurons of the peripheral nervous system and forebrain cholinergic neurons. NGF promotes the development of neurite-like filaments from chick embryo dorsal root ganglia and rat PC12 pheochromocytoma cells. NGF may have a role in in vivo fetal development and nerve regeneration especially, after ischemic, surgical, or chemical injuries. NGF may also be associated with the central nervous system. It exhibits ameliorating effects against ulcers (human cutaneous, corneal ulcers, and pressure ulcers), glaucoma, maculopathy, and retinitis pigmentosa. NGF-2.5S is a type of NGF-b originally derived under dissociative conditions and often varies from NGF-b due to proteolysis arising during its purification.
Other Notes
2.5S subunit of nerve growth factor-7S (NGF-7S) is essentially the β-subunit when isolated from male mouse submaxillary glands under initially dissociative conditions by a modification of the method of Bocchini and Angeletti.
Physical form
Lyophilized from a 0.2 μm filtered solution in sodium acetate buffer.
Analysis Note
The biological activity is measured using a 3-day MTT assay.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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FASEB bioAdvances, 4(1), 76-89 (2022-01-14)
Tumor innervation has recently been documented and characterized in various settings and tumor types. However, the role that nerves innervating tumors play in the pathogenesis of cancer has not been clarified. In this study, we searched for neural signaling from
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Dental pulp stem cells (DPSCs) secrete neurotrophic factors which may play an important therapeutic role in neural development, maintenance and repair. To test this hypothesis, DPSCs-conditioned medium (DPSCs-CM) was collected from 72 hours serum-free DPSCs cultures. The impact of DPSCs-derived
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Methods in molecular biology (Clifton, N.J.), 2311, 177-184 (2021-05-26)
Preparations of peripheral sensory neurons from rodents are essential for studying the molecular mechanism of neuronal survival and physiology. Although, isolating and culturing these neurons proves difficult, often these preparations are contaminated with nonneuronal proliferating cells. Here, we describe an
Antonio Merolli et al.
Journal of anatomy, 240(5), 998-1001 (2021-11-24)
Fluorescent markers, generally targeting neurotubules, are used to visualize the radiating crown of growing neurites that is produced by dorsal root ganglion cells in vitro. Hoechst 33342 (2'-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5'-bi-1H-benzimidazole trihydrochloride trihydrate) is a widely used fluorescent DNA marker that stain both
Hidetada Matsuoka et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 68(10), 679-690 (2020-09-05)
TWIK-related acid-sensitive K+ (TASK) homomeric channels, TASK1 and TASK3, are present in PC12 cells. The channels do not heteromerize due plausibly to a lack of p11 protein. Single-channel recording reveals that most of the rat carotid body (CB) glomus cells
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