N5415
Nerve Growth Factor-ß human
≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture
Synonym(s):
β-NGF, BNGF, NGF-β, NGF-beta
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.77
Product Name
Nerve Growth Factor-ß human, recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture
biological source
human
recombinant
expressed in HEK 293 cells
assay
≥95% (SDS-PAGE)
form
lyophilized powder
potency
≤16 ng/mL ED50/EC50
mol wt
protein calculated mol wt 13.5 kDa (Monomer)
packaging
pkg of 10 μg
pkg of 100 μg
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/μg Endotoxin (β-NGF)
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
human ... NGF(4803)
Analysis Note
The specific activity is determined by the dose dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
Biochem/physiol Actions
A mutation in the NGF-β gene is implicated in the loss of pain perception.
Nerve growth factor β (β-NGF) is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. β-NGF promotes the survival and cholinergic phenotype of basal forebrain cholinergic neurons and stimulates neurite outgrowth of embryonic trigeminal motor neurons in vitro.
Disclaimer
The product retains activity for at least 2 years as supplied. After initial thawing it is recommended to store the protein in working aliquots at -20 °C.
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
NGF-β gene is mapped to human chromosome 1p13.2.
Recombinant human β-NGF is expressed in human 293 cells as a homodimer (non-disulfide bonded) of 120 amino acids, with a calculated molecular weight of 13.5 kDa (monomer). Nerve growth factor β (β-NGF) is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. The production of natural β-NGF occurs in epithelial cells, and the pituitary gland.
Physical form
This product is lyophilized from PBS.
Preparation Note
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile DDW containing 0.1% endotoxin-free recombinant human serum albumin, to a final concentration of 100 μg/ml.
Store product at –20 °C. The product retains activity for at least 2 years as supplied. After initial thawing it is recommended to store the protein in working aliquots at –20 °C.
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 1
Regulatory Information
监管及禁止进口产品
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Yousong Su et al.
Progress in neuro-psychopharmacology & biological psychiatry, 104, 110015-110015 (2020-06-23)
The neurodevelopmental hypothesis of schizophrenia has been widely accepted. In light of our previous microarray data, two neurodevelopment-related genes were focused on inclduing the N-acylsphingosine amidohydrolase 1 gene (ASAH1) and the nerve growth factor gene (NGF). The evidence that ASAH1
Elisabet Einarsdottir et al.
Human molecular genetics, 13(8), 799-805 (2004-02-21)
Identification of genes associated with pain insensitivity syndromes can increase the understanding of the pathways involved in pain and contribute to the understanding of how sensory pathways relate to other neurological functions. In this report we describe the mapping and
S Soinila et al.
Neuroscience research, 6(2), 123-130 (1988-12-01)
Nerve growth factor (NGF)-like immunoreactivity was demonstrated in the rat pituitary intermediate lobe by indirect immunofluorescence method using antisera specific to beta-NGF isolated from adult male mouse submaxillary salivary gland. Co-culture of frozen or fresh intermediate lobes with newborn rat
Katherine M Nielsen et al.
Molecular and cellular neurosciences, 25(4), 629-641 (2004-04-15)
Developing neurons encounter a panoply of extracellular signals as they differentiate. A major goal is to identify these extrinsic cues and define the mechanisms by which neurons simultaneously integrate stimulation by multiple factors yet initiate one specific biological response. Factors
A Rosenthal et al.
Handbook of experimental pharmacology, 220, 497-512 (2014-03-29)
The neurotrophin family is comprised of the structurally related secreted proteins nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophine-4 (NT-4). They bind and activate the tyrosine kinase receptors Trk A, B, and C in a ligand-specific
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