Skip to Content
Merck
CN
All Photos(2)

Key Documents

View All Documentation

Safety Information

N2643

Sigma-Aldrich

Anti-Nitric Oxide Synthase, Endothelial (596-609) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-eNOS

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

MDL number:
MFCD01092429
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human, mouse, bovine

technique(s)

immunohistochemistry (frozen sections): 1:100 using acetone-fixed, frozen tissue sections of mouse heart
microarray: suitable
western blot: 1:10,000 using bovine lung endothelial cell extract

UniProt accession no.

P29474

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NOS3(4846)
mouse ... Nos3(18127)

General description

The gene encoding endothelial nitric oxide synthase (eNOS) is localized on human chromosome 7q35-36 and has 26 exons.
eNOS encodes a 135 kDa protein and is localized in endothelial cells. Human bNOS and eNOS share 52% amino acid identity, and rat and human bNOS share 93% amino acid identity. eNOS is not restricted to the endothelium of blood vessels but exists in the epithelium of several tissues.

Immunogen

synthetic peptide corresponding to nitric oxide synthase (NOS) of bovine endothelial origin (eNOS, amino acids 596-609) conjugated to KLH. The immunogen sequence is identical in human eNOS.

Application

Anti-Nitric Oxide Synthase, Endothelial (596-609) antibody produced in rabbit has been used in western blotting.

Biochem/physiol Actions

Endothelial nitric oxide synthase (eNOS) is a modulator of vascular function and it is involved in the production of nitric oxide. Defects in the enzyme expression have been shown to be associated coronary artery disease.
Endothelial nitric oxide synthase (eNOS) is constitutively expressed and are dependent on Ca2+/ calmodulin for nitricoxide (NO) production.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
127K4864
107K4782
114K4836
057H4842
024K4854

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Intracellular processing of endothelial nitric oxide synthase isoforms associated with differences in severity of cardiopulmonary diseases: cleavage of proteins with aspartate vs. glutamate at position 298
Tesauro M, et al.
Proceedings of the National Academy of Sciences of the USA, 97(6), 2832-2835 (2000)
Nitric oxide production by mouse renal tubules can be increased by a sodium-dependent mechanism
Kempson S, et al.
Nitric Oxide, 17(1), 33-43 (2007)
Vascular nitric oxide: Beyond eNOS
Zhao Y, et al.
Journal of Pharmacological Sciences, 129(2), 83-94 (2015)
Ang-(1-7) protects HUVECs from high glucose-induced injury and inflammation via inhibition of the JAK2/STAT3 pathway
Chen J, et al.
International Journal of Molecular Medicine, 41(5), 2865-2878 (2018)
Molecular basis of cell-specific endothelial nitric-oxide synthase expression in airway epithelium
German Z, et al.
The Journal of Biological Chemistry, 275(11), 8183-8189 (2000)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service