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N1142

Sigma-Aldrich

Neomycin

solution, suitable for cell culture, BioReagent

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Synonym(s):
Antibiotic 10676, Neomycin B
UNSPSC Code:
12352207
PubChem Substance ID:
24897464
NACRES:
NA.76

product name

Neomycin solution, with 10 mg/mL neomycin in 0.9% NaCl, 0.1 μm filtered, BioReagent, suitable for cell culture

biological source

Streptomyces fradiae

Quality Level

200

sterility

0.1 μm filtered

product line

BioReagent

form

liquid

potency

8-12 mg per mL

packaging

pkg of 20ML

concentration

10 mg/mL in 0.9% NaCl (neomycin)

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

clear

UniProt accession no.

O14746

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria

Mode of action

protein synthesis | interferes

storage temp.

2-8°C

SMILES string

NC[C@H]1O[C@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O)[C@H]2O[C@@H]3O[C@H](CO)[C@@H](O[C@H]4O[C@@H](CN)[C@@H](O)[C@H](O)[C@H]4N)[C@H]3O)[C@H](N)[C@@H](O)[C@@H]1O

Gene Information

human ... TERT(7015)

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General description

Chemical structure: aminoglycoside

Application

Neomycin solution has been used:
  • as a supplement of autoclaved acidified water for bone marrow transplantation
  • for the selection of stable clones
  • as a component of artificial sea water (ASWO) medium to remove bacteria from O. tauri cultures

Biochem/physiol Actions

Neomycin is an aminoglycoside antibiotic, produced by Streptomyces containing a minimum of 85% neomycin B. It acts as a selection agent for prokaryotic cells transformed using the neo selectable marker gene. Neomycin trisulfate is used to study the cytototoxic side effects of antibiotics, platelet-derived growth factor responses in certain fibroblasts and extraction of nuclear phosphatidylinositol 4,5-bisphosphate-interacting proteins. This product is recommended for use in cell culture applications at 5 mL/L.
Mode of action: This product acts by binding to the 30S and 50S subunits, causing miscoding and inhibiting initiation and elongation during protein synthesis. Neomycin also blocks voltage-sensitive Ca2+ channels without affecting the Na+/Ca2+ antiporter in neurons.
Antimicrobial spectrum: Neomycin acts against both gram-positive and gram-negative bacteria

Caution

This solution is formulated to contain 10 mg/mL neomycin in 0.9% sodium chloride. It is stable at 37 °C for 5 days and should be otherwise stored at 2-8°C.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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