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MAK264

Sigma-Aldrich

High Sensitivity Triglyceride Fluorometric Assay Kit

sufficient for 100 fluorometric tests

Synonym(s):

Triglyceride Quantification Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

application(s)

cosmetics
food and beverages

detection method

fluorometric

relevant disease(s)

neurological disorders; cardiovascular diseases

storage temp.

−20°C

General description

Triglycerides (TG) are the main constituents of vegetable oil, animal fat, LDL and VLDL, and play an important role as transporters of fatty acids as well as an energy source. TG is broken down into fatty acids and glycerol, after which both can serve as substrates for energy producing and metabolic pathways. High blood levels of TG are implicated in atherosclerosis, heart disease and stroke as well as in pancreatitis.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for measuring triglyceride levels in serum, plasma, saliva, and other biological fluids, and tissue and cell culture samples.

Principle

The High Sensitivity Triglyceride Assay Kit is suitable for measuring triglyceride levels in samples that contain reducing substances that may interfere with oxidase-based assays. In this assay, TG is hydrolyzed with lipase to glycerol and fatty acids. The glycerol is used in a coupled enzyme reaction, which results in a fluorometric product (λex = 535 nm/λem = 587 nm), proportional to the amount of triglycerides present. This high-throughput, adaptable assay kit is simple and sensitive with the capability to detect less than 0.4 μM of triglycerides in a variety of samples.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Diet and Health (1989)
Triglycerides and cardiovascular disease.
Nordestgaard B G and Varbo A
Lancet, 384(9943), 626-635 (2014)
Ablation of adipocyte creatine transport impairs thermogenesis and causes diet-induced obesity.
Lawrence Kazak et al.
Nature metabolism, 1(3), 360-370 (2019-06-05)
New insights into the molecular mechanism of intestinal fatty acid absorption.
Wang T Y, et al.
European Journal of Clinical Investigation, 43(11), 1203-1223 (2013)
Chun-Che Lin et al.
Iranian journal of basic medical sciences, 24(8), 1146-1152 (2021-11-23)
Protective effects of s-methyl cysteine (SMC) alone, protocatechuic acid (PCA) alone, and SMC plus PCA against chronic ethanol consumption induced hepatic steatosis and inflammation were investigated. Mice were divided into six groups: normal diet (ND) group, Lieber-DeCarli liquid diet without

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