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Merck
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MAK117

Glycerol Assay Kit

sufficient for 200 colorimetric or fluorometric tests

Synonym(s):

Glycerin Assay Kit, Glycerine Assay Kit

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About This Item

NACRES:
NA.84
UNSPSC Code:
12161503

Key Documents

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Product Name

Glycerol Assay Kit, sufficient for 200 colorimetric or fluorometric tests

usage

sufficient for 200 colorimetric or fluorometric tests

species reactivity

Drosophila, human, mouse

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

activity assay: suitable

input

food(s)
beverage(s)
sample(s)

accession no.

NM_000167.6
NM_001273184.2
P13706
P32189

application(s)

cosmetics
environmental
food and beverages

detection method

colorimetric
fluorometric

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−20°C

Gene Information

Drosophila ... Gpdh1(33824)
human ... GK(2710 )
mouse ... Gpd1(14555)

Application

Glycerol assay kit has been used to quantify glycerol release from mouse 3T3-L1 cell culture supernatant samples and to measure plasma glycerol levels.
This kit is suitable for glycerol detection in foods, beverages, pharmaceutical formulations, and biological samples.

Biochem/physiol Actions

In this kit, glycerol concentration is determined by a coupled enzyme assay involving glycerol kinase and glycerol phosphate oxidase, resulting in a colorimetric (570 nm)/fluorometric (lex = 535/lem = 587 nm) product, proportional to the glycerol present. The linear range of detection for this kit is 10-1,000 μM (colorimetric) and 2-50 μM (fluorometric).

Features and Benefits

Compatible with high-throughput handling systems.

General description

Glycerol, also referred to as glycerin or glycerine, is a 3-carbon sugar alcohol that forms the backbone of fatty acids. It is also a component of phospholipids and triglycerides. Glycerol is a sweet-tasting, hygroscopic liquid. Glycerol is widely used in food, beverage, and pharmaceutical formulations and is the main waste by-product of biodiesel production via transesterification.

Storage Class

10 - Combustible liquids

Regulatory Information

常规特殊物品
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Certificates of Analysis (COA)

Lot/Batch Number
117CF08A22
117CF08A22
117CF07A09
117CF07A09
117CF04A04

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