MAK092
Peroxidase Activity Assay Kit
sufficient for 100 colorimetric or fluorometric tests
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
usage
sufficient for 100 colorimetric or fluorometric tests
detection method
colorimetric
fluorometric
storage temp.
−20°C
General description
Peroxidase (EC 1.11.1.7) is an enzyme found broadly in biological systems that utilizes hydrogen peroxide in the oxidation of various substrates. The Peroxidase Activity Assay Kit provides a simple and direct procedure for measuring peroxidase activity in a variety of biological samples.
Application
Peroxidase Activity Assay Kit has been used to quantify the activity of peroxidase.
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for the determination of peroxidase activity in cell culture supernatant, serum, plasma, urine, and other biological fluids.
Principle
Peroxidase catalyzes the reaction between H2O2 and the probe, resulting in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the peroxidase activity present. One unit of peroxidase is defined as the amount of enzyme that reduces1.0 μmole of H2O2 per minute at 37°C.
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Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
10 - Combustible liquids
Flash Point(F)
188.6 °F - closed cup
Flash Point(C)
87 °C - closed cup
Regulatory Information
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4.09 - Peroxidases
Charlene A M
Comprehensive Toxicology, 4, 169-184 (2010)
Agnieszka Kiełbowicz-Matuk et al.
Frontiers in plant science, 13, 965098-965098 (2022-09-27)
The transition from vegetative growth to reproductive development is a critical developmental switch in flowering plants to ensure a successful life cycle. However, while the genes controlling flowering are well-known in model plants, they are less well-understood in crops. In
Sujitha Balakrishnan Sulochana et al.
Frontiers in bioengineering and biotechnology, 8, 585632-585632 (2020-11-17)
Scenedesmus quadricauda CASA CC202, a potent freshwater microalga is being used as a biofuel feedstock, which accumulates 2.27 fold lipid during nitrogen stress induction. Upon nitrogen starvation, S. quadricauda undergoes biochemical and metabolic changes that perturb the cell to cope
Prasad Kottayil Padmanabhan et al.
Cell death & disease, 7(10), e2406-e2406 (2016-10-14)
DDX3 is a highly conserved member of ATP-dependent DEAD-box RNA helicases with multiple functions in RNA metabolism and cellular signaling. Here, we describe a novel function for DDX3 in regulating the mitochondrial stress response in the parasitic protozoan Leishmania. We
Microscopic and molecular characterization of the prehaustorial resistance against wheat leaf rust (Puccinia triticina) in Einkorn (Triticum monococcum).
Serfling A, et al.
Frontiers in Plant Science, 7, 1668-1668 (2016)
Protocols
This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate.
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