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MAK083
Glucose Uptake Colorimetric Assay Kit
sufficient for 100 colorimetric tests
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1 KIT
CN¥18,055.43
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1 KIT
CN¥18,055.43
About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
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usage
sufficient for 100 colorimetric tests
detection method
colorimetric
relevant disease(s)
endocrinological disorders, diabetes; cancer
storage temp.
−20°C
1 of 4
This Item | MAK084 | MAK263 | MAK098 |
|---|---|---|---|
| detection method colorimetric | detection method fluorometric | detection method colorimetric, fluorometric | detection method colorimetric |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| relevant disease(s) endocrinological disorders, diabetes; cancer | relevant disease(s) endocrinological disorders, diabetes; cancer | relevant disease(s) endocrinological disorders, diabetes | relevant disease(s) cancer |
General description
Glucose is the primary source of energy for most cells. Glucose uptake into cells is highly regulated and the first rate limiting step in glucose metabolism. Glucose uptake is facilitated by the GLUT family of transporter proteins, whose expression and activity are regulated by multiple mechanisms. Glucose uptake is upregulated in many cancer cells, which exhibit high rates of aerobic glycolysis. Cells exhibiting insulin resistance show diminished glucose uptake in response to insulin stimulation.
The use of the recycling amplification reaction in the colorimetric assay results in the limit of detection being 10-fold lower (20-100 pmole) compared to the fluorescence assay (200-1,000 pmole, MAK084).
The use of the recycling amplification reaction in the colorimetric assay results in the limit of detection being 10-fold lower (20-100 pmole) compared to the fluorescence assay (200-1,000 pmole, MAK084).
Application
Suitability
Suitable for detecting glucose uptake in adherent or suspension cells cultured in a 96-well microtiter plate.
Principle
The Glucose Uptake Colorimetric Assay kit provides a simple and direct procedure for measuring glucose uptake in a variety of cells. Glucose uptake is measured using the glucose analog, 2-deoxyglucose (2-DG), which is taken up by cells and phosphorylated by hexokinase to 2-DG6P. 2-DG6P cannot be further metabolized and accumulates in cells, directly proportional to the glucose uptake by cells. In this assay, 2-DG uptake is determined by a coupled enzymatic assay in which the 2-DG6P is oxidized, resulting in the generation of NADPH, which is then determined by a recycling amplification reaction in which the NADPH is utilized by glutathione reductase in a coupled enzymatic reaction that produces glutathione. Glutathione reacts with DTNB to product TNB, which is detected at 412 nm.
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