MAK098
Glucose-1-Phosphate (G1P) Colorimetric Assay Kit
sufficient for 100 colorimetric tests
Synonym(s):
G1P Quantification Colorimetric Assay Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
usage
sufficient for 100 colorimetric tests
detection method
colorimetric
relevant disease(s)
cancer
storage temp.
−20°C
General description
Glucose-1-phosphate (G1P), also known as the Cori ester due to its discovery by Gerty and Carl Cori, is produced from glycogen during glycogenolysis by the actions of glycogen phosphorylase. Conversion to glucose-6-phosphate (G6P) by phosphoglucomutase allows for entry of the glucose molecule into metabolic pathways such as glycolysis. During glycogenesis, G6P is converted to G1P by the actions of phosphoglucose isomerase.
Suitability
Suitable for the measurement of glucose-1-phosphate in various tissues (liver, muscle and heart etc.) and cells (adherent or suspension), the analysis of glucose metabolism and cell signaling in various cells and screening anti-diabetic drugs.
Principle
The G1P Colorimetric Assay kit provides a simple and direct procedure for measuring G1P in a variety of samples. G1P concentration is determined by a coupled enzyme assay, in which G1P is converted to glucose-6-phosphate by phosphoglucomutase in the presence of glucose-1,6-biphosphate. The glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH. The resulting NADH reduces a colorless probe resulting in a colorimetric (450 nm) product proportional to the G1P present.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
10 - Combustible liquids
Regulatory Information
监管及禁止进口产品
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Dong-Xue Ding et al.
iScience, 25(3), 103955-103955 (2022-03-15)
MYCT1 has been shown to function as a tumor suppressor in various tumors, but its role in metabolism has never been reported. Here, we showed that global inactivation of Myct1 in mice led to progressive accumulation of glycogen in the
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