MAK051
Aconitase Activity Assay Kit
sufficient for 100 colorimetric tests
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Synonym(s):
Aconitase Enzyme Activity Kit
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
usage
sufficient for 100 colorimetric tests
detection method
colorimetric
relevant disease(s)
neurological disorders; cardiovascular diseases; cancer
storage temp.
2-8°C
Gene Information
human ... ACO1(48) , ACO2(50)
mouse ... ACO1(11428) , ACO2(11429)
rat ... ACO1(50655) , ACO2(79250)
General description
The aconitases are a family of iron-sulfur containing enzymes that catalyzes the isomerization of citrate to isocitrate. Two aconitases, encoded by different genes, have been identified in eukaryotes. Aconitase 1 (ACO1, c-aconitase) is a cytosolic enzyme while Aconitase 2 (ACO2, m-aconitase) is a mitochondrial enzyme that functions in the tricarboxylic acid cycle. Aconitases are reversibly inactivated by oxidative stress, and aconitase activity in cells and tissues has been used as a biomarker for oxidative damage.
Application
Aconitase Activity Assay Kit has been used to measure the activity of cytosolic and mitochondrial aconitase enzyme.
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for the measurement of aconitase activity in biological samples including tissue and cells
Principle
The Aconitase Activity Assay kit provides a simple and direct procedure for measuring Aconitase activity in a variety of samples. Aconitase activity is determined in a coupled enzyme reaction in which citrate is converted to isocitrate by aconitase. This results in a colorimetric (450 nm) product proportional to the enzymatic activity present. One unit of aconitase is the amount of enzyme that will isomerize 1.0 μmole of citrate from isocitrate per minute at pH 7.4 at 25 °C.
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Danger
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Hazard Classifications
Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B
WGK
WGK 3
Regulatory Information
动植物源性产品
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Jayasimman Rajendran et al.
EMBO molecular medicine, 11(1) (2018-12-12)
Alternative oxidase (AOX) is a non-mammalian enzyme that can bypass blockade of the complex III-IV segment of the respiratory chain (RC). We crossed a Ciona intestinalis AOX transgene into RC complex III (cIII)-deficient Bcs1l
Vincenzo Maione et al.
Biochimica et biophysica acta. General subjects, 1862(9), 1980-1987 (2018-05-31)
The CIA2A protein, in complex with CIAO1, has been proposed to be exclusively implicated in the maturation of cytosolic aconitase. However, how the CIA2A-CIAO1 complex generates active aconitase is still unknown and the available structural information has not provided any
Evaluation of the adaptogenic potential exerted by ginsenosides Rb1 and Rg1 against oxidative stress-mediated neurotoxicity in an in vitro neuronal model.
Carlos F M, et al.
PLoS ONE, 12(8), e0182933-e0182933 (2017)
François Beaufay et al.
mBio, 11(4) (2020-07-30)
Maintaining cellular iron homeostasis is critical for organismal survival. Whereas iron depletion negatively affects the many metabolic pathways that depend on the activity of iron-containing enzymes, any excess of iron can cause the rapid formation of highly toxic reactive oxygen
NFS1 undergoes positive selection in lung tumours and protects cells from ferroptosis.
Alvarez S W, et al.
Nature, 551(7682), 639-639 (2017)
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