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About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
usage
sufficient for 100 colorimetric or fluorometric tests
detection method
colorimetric, fluorometric
storage temp.
−20°C
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General description
Glycogen is a branched polymer of glucose that serves as the primary short-term energy storage molecule in animals. Glycogen is primarily synthesized in liver and muscle tissue where it can constitute up to 10% of the weight of liver and 1-2% of the weight of muscle tissue. While muscle glycogen is generally utilized locally, liver glycogen serves as an important buffer to regulate blood glucose levels. Glycogen metabolism is dysregulated in diabetes and the glycogen storage diseases due to inborn errors of metabolism.
Application
Glycogen Assay Kit has been used in glycogen quantification. It has also been used to determine the glycogen content in liver, vastus lateralis and muscle homogenates.
Suitable for determining glycogen concentration in various tissues such as liver etc. and cell culture (adherent or suspension cells).
Biochem/physiol Actions
Glycogen concentration is determined by a coupled enzyme assay, which produces a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the glycogen present.
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
10 - Combustible liquids
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
Regulatory Information
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Cassius E O Coombs et al.
Meat science, 134, 86-97 (2017-08-05)
This study evaluated the effect of chilled followed by frozen storage on lamb quality and safety parameters. Experimental (n=360) M. longissimus lumborum (LL) were randomly sampled from the boning room of a commercial Australian abattoir, at 24 h post-mortem, and
Madhuri S Salker et al.
Scientific reports, 7(1), 12612-12612 (2017-10-05)
Embryo implantation requires a hospitable uterine environment. A key metabolic change that occurs during the peri-implantation period, and throughout early pregnancy, is the rise in endometrial glycogen content. Glycogen accumulation requires prior cellular uptake of glucose. Here we show that
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Journal of translational medicine, 14(1), 344-344 (2016-12-22)
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Fettiplace M R, et al.
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Hosts of chemoautotrophic bacteria typically have much higher biomass than their symbionts and consume symbiont cells for nutrition. In contrast to this, chemoautotrophic Candidatus Riegeria symbionts in mouthless Paracatenula flatworms comprise up to half of the biomass of the consortium.
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