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Merck
CN

MAK016

Glycogen Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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About This Item

NACRES:
NA.84
UNSPSC Code:
12161503
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usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric, fluorometric

storage temp.

−20°C

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General description

Glycogen is a branched polymer of glucose that serves as the primary short-term energy storage molecule in animals. Glycogen is primarily synthesized in liver and muscle tissue where it can constitute up to 10% of the weight of liver and 1-2% of the weight of muscle tissue. While muscle glycogen is generally utilized locally, liver glycogen serves as an important buffer to regulate blood glucose levels. Glycogen metabolism is dysregulated in diabetes and the glycogen storage diseases due to inborn errors of metabolism.

Application

Glycogen Assay Kit has been used in glycogen quantification. It has also been used to determine the glycogen content in liver, vastus lateralis and muscle homogenates.
Suitable for determining glycogen concentration in various tissues such as liver etc. and cell culture (adherent or suspension cells).

Biochem/physiol Actions

Glycogen concentration is determined by a coupled enzyme assay, which produces a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the glycogen present.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

Regulatory Information

常规特殊物品
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Cassius E O Coombs et al.
Meat science, 134, 86-97 (2017-08-05)
This study evaluated the effect of chilled followed by frozen storage on lamb quality and safety parameters. Experimental (n=360) M. longissimus lumborum (LL) were randomly sampled from the boning room of a commercial Australian abattoir, at 24 h post-mortem, and
Madhuri S Salker et al.
Scientific reports, 7(1), 12612-12612 (2017-10-05)
Embryo implantation requires a hospitable uterine environment. A key metabolic change that occurs during the peri-implantation period, and throughout early pregnancy, is the rise in endometrial glycogen content. Glycogen accumulation requires prior cellular uptake of glucose. Here we show that
Nady Golestaneh et al.
Journal of translational medicine, 14(1), 344-344 (2016-12-22)
Study of age related macular degeneration (AMD) has been hampered by lack of human models that represent the complexity of the disease. Here we have developed a human in vitro disease model of AMD to investigate the underlying AMD disease
Insulin Signaling in Bupivacaine-induced Cardiac ToxicitySensitization during Recovery and Potentiation by Lipid Emulsion
Fettiplace M R, et al.
Anesthesiology, 124(2), 428-\442-428-\442 (2016)
Long-term obestatin treatment of mice type 2 diabetes increases insulin sensitivity and improves liver function.
Kolodziejski P A, et al.
Endocrine, 56(3), 538-550 (2017)

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