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M9570

Sigma-Aldrich

Anti-Matrix Metalloproteinase-9 antibody produced in goat

affinity isolated antibody

Synonym(s):

MMP-9 Antibody - Anti-Matrix Metalloproteinase-9 antibody produced in goat, Mmp-9 Antibody, Anti-MMP-9

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

goat

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse

technique(s)

immunohistochemistry: 5-15 μg/mL using cells or tissues
immunoprecipitation (IP): 25 μg/mL using conditioned media of transfected NSO cells
western blot: 0.25 μg/mL

UniProt accession no.

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Mmp9(17395)

General description

Matrix metalloproteinase-9 (MMP-9) is a pro-inflammatory, zinc-dependent proteinase which is also known as 92kDa gelatinase. It is produced by various cells like granulocytes and mononuclear cells. The gene encoding it is localized on human chromosome 20q11.2-q13.1.

Specificity

This antibody recognizes pro and active mouse MMP-9. Immunoblotting and ELISA applications show an ~10% cross-reactivity with recombinant human MMP-9.

Immunogen

purified, NSO-derived, recombinant mouse matrix metalloproteinase-9.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

Biochem/physiol Actions

Matrix metalloproteinase-9 (MMP-9) has been studied as a biomarker of nervous tissue inflammation in multiple sclerosis (MS).

Target description

Matrix Metalloproteinase-9 encodes an enzyme that degrades type IV and V collagens.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Cécile Cléry-Barraud et al.
Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI), 19(1), e146-e156 (2012-06-30)
To date, sulphur mustard (SM) cutaneous toxicity has been commonly assessed on account of several animal models such as pigs and weanling pigs. Few experiments however, have been carried out on mice so far. In this study, we aimed at
Yuta Morisaki et al.
Scientific reports, 6, 27354-27354 (2016-06-07)
Differential vulnerability among motor neuron (MN) subtypes is a fundamental feature of amyotrophic lateral sclerosis (ALS): fast-fatigable (FF) MNs are more vulnerable than fast fatigue-resistant (FR) or slow (S) MNs. The reason for this selective vulnerability remains enigmatic. We report
Hidemi Misawa et al.
Genesis (New York, N.Y. : 2000), 54(11), 568-572 (2016-09-07)
VAChT-Cre.Fast and VAChT-Cre.Slow mice selectively express Cre recombinase in approximately one half of postnatal somatic motor neurons. The mouse lines have been used in various studies with selective genetic modifications in adult motor neurons. In the present study, we crossed
Xiaoli Zhang et al.
Medical science monitor : international medical journal of experimental and clinical research, 21, 1115-1123 (2015-04-22)
The role of matrix metalloproteinase 9 (MMP-9) polymorphisms in breast cancer risk remains unclear. The purpose of this study was to evaluate the association between MMP-9 variants and breast cancer susceptibility. Case-control studies were searched on electronic databases to retrieve
Brigid K Jensen et al.
Glia, 70(7), 1426-1449 (2022-04-28)
Genetic mutations that cause amyotrophic lateral sclerosis (ALS), a progressively lethal motor neuron disease, are commonly found in ubiquitously expressed genes. In addition to direct defects within motor neurons, growing evidence suggests that dysfunction of non-neuronal cells is also an

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