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M8945

Sigma-Aldrich

Matrix Metalloproteinase-9 human

recombinant, >90% (SDS-PAGE), buffered aqueous solution

Synonym(s):

Gelantinase-B, Gelatinase, 95 kDa, MMP-9

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About This Item

MDL number:
MFCD01323840
UNSPSC Code:
12352204
NACRES:
NA.32

Quality Level

200

Assay

>90% (SDS-PAGE)

form

buffered aqueous solution

mol wt

apparent mol wt ~93 kDa

UniProt accession no.

P14780

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... MMP9(4318)

Biochem/physiol Actions

MMP-9 degrades the collagens that make up the extracellular matrix, which has a role in the control of angiogenesis.

Physical form

Solution in Tris-HCl, pH 7.5, Calcium Chloride, Sodium Chloride, and Brij-35

Analysis Note

The biological activity is measured by its ability to cleave a fluorogenic peptide substrate.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the molecular weight of product M8945 (Matrix Metalloproteinase-9 Human)

    Human MMP-9 consists of a 688 amino acid residue recombinant protein which has a predicted molecular mass of approximately 77 kDa. By SDS-PAGE the apparent molecular mass is approximately 93 kDa.

  6. Does product M8945 (Matrix Metalloproteinase-9 Human) need to be activated?

    To activate recombinant human MMP-9, prepare a p-aminophenylmercuric acetate (APMA) solution in DMSO. Add the AMPA solution to the MMP-9 to a final APMA concentration of 1 mM. Incubate at 37°C. for 16 - 24 hours.

  7. Is product M8945 (Marix Metalloproteinase-9 Human) endotoxin free?

    This product contains < 1.0 EU/ug of the enzyme.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Carla Graça et al.
Foods (Basel, Switzerland), 9(10) (2020-10-07)
The influence of flour replacement by yogurt or curd-cheese additions (from 10% to 20%, w/w) on the glycemic response and bioactivity improvements of gluten-free bread was evaluated. Starch digestibility, measured by an in vitro digestion model, was applied to determine
Gelatinase B
Collier, I.E., and Goldberg, G.I. et al.
Handbook of Proteolytic Enzymes, 1205-1210 (1998)
S Chandler et al.
Neuroscience letters, 201(3), 223-226 (1995-12-15)
Matrix metalloproteinases (MMPs) are a group of enzymes responsible for the degradation of interstitial connective tissue and basement membrane. The coding sequences for five of the human MMPs, viz. interstitial collagenase, 72 kDa gelatinase, stromelysin-1, matrilysin and 92 kDa gelatinase
Marcelo Rodríguez-Piñón et al.
Anatomia, histologia, embryologia, 48(4), 306-314 (2019-03-25)
The tortuous nature of the ovine cervix restricts the transcervical passage of the cannula, and many studies have aimed to understand the endocrine mechanism of the remodelling of cervical tissue in adult ewe. However, little is known about the remodelling
Wei-Li Hsu et al.
BMC veterinary research, 10, 202-202 (2014-08-28)
Neutrophil gelatinase-associated lipocalin (NGAL) is a useful biomarker for the early prediction of renal diseases. NGAL may exist as monomer, dimer and/or NGAL/MMP-9 complex forms in humans. In this study, the existence of various forms of NGAL in urine (uNGAL)
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