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M8320

Sigma-Aldrich

Anti-MLH1 (N-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

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Synonym(s):
Anti-COCA2, Anti-FCC2, Anti-HNPCC, Anti-MGC5172
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 80-85 kDa

species reactivity

rat, human, mouse

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

immunocytochemistry: 2.5-5 μg/mL using MCF7 cells fixed with paraformaldehyde-Triton
immunoprecipitation (IP): 5-10 μg using Jurkat cell lysates
western blot: 0.5-1 μg/mL using Jurkat cell lysates

UniProt accession no.

P40692

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MLH1(4292)
mouse ... Mlh1(17350)
rat ... Mlh1(81685)

General description

MutL homolog 1 (MLH1) is a nucleoprotein and a major component of mismatch repair system. The MLH1 gene is localized on chromosome 3p21 and is made up of 19 exons. The protein has a molecular weight of 80kDa.
MutL homolog 1 (MLH1) is part of a large multi-subunit protein complex of tumor suppressors, DNA damage sensors and signal transducers, named BRCA1-associated genome surveillance complex (BASC).

Immunogen

synthetic peptide corresponding to amino acids 60-75 of human MLH1, conjugated to KLH via an N-terminal added cysteine residue. The corresponding peptide sequence is conserved in human, rat, and mouse.

Application

  • Anti-MLH1 (N-terminal) antibody produced in rabbit has been used in:
  • immunoblotting
  • immunoprecipitation
  • immunocytochemistry

Biochem/physiol Actions

MutL homolog 1 (MLH1) has been shown to be involved in stimulating carcinogenesis in the colon.
MutL protein (MLH), a homolog of the E. coli MutL gene, is involved in DNA mismatch repair. Nonpolyposis colorectal cancer-2 is caused by a hereditary mutation in the MLH1 gene. This cancer can also be a result of hypermethylation of one MLH1 allele in somatic cells (a germline epimutation).

Target description

MLH1 (N-terminal) is part of a large multi-subunit protein complex of tumor suppressors, DNA damage sensors, and signal transducers, named BASC (BRCA1-associated genome surveillance complex).

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Hereditary nonpolyposis colorectal cancer (HNPCC)/Lynch syndrome
Steinke V. et al.
Deutsches Arzteblatt International, 110(3), 32-32 (2013)
BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures
Wang Y, et al.
Genes & Development, 14(8), 927-939 (2000)
Haiyan Chen et al.
Journal of cancer research and clinical oncology, 141(12), 2147-2158 (2015-05-20)
As one of the most essential components of mismatch repair system, MutL homolog 1 (MLH1) plays an increasingly implicated role in initiation and promotion of colorectal carcinogenesis, with germ-line mutations in different loci. However, whether a single genetic variant in
Shinichiro Fukuhara et al.
Oncotarget, 5(22), 11297-11307 (2014-12-20)
Mismatch repair (MMR) enzymes have been shown to be deficient in prostate cancer (PCa). MMR can influence the regulation of tumor development in various cancers but their role on PCa has not been investigated. The aim of the present study

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