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M3671

Sigma-Aldrich

MES

low moisture content, ≥99% (titration)

Synonym(s):

2-(N-Morpholino)ethanesulfonic acid, 4-Morpholineethanesulfonic acid

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About This Item

Empirical Formula (Hill Notation):
C6H13NO4S
CAS Number:
4432-31-9
Molecular Weight:
195.24
Beilstein:
141319
EC Number:
224-632-3
MDL number:
MFCD00006181
UNSPSC Code:
12161700
eCl@ss:
32129211
PubChem Substance ID:
24896867
NACRES:
NA.25

Quality Level

400

Assay

≥99% (titration)

form

powder

storage condition

dry at room temperature

impurities

≤1.0% water

color

white

pH

2.5-4 (25 °C, 97.6 g/L)

useful pH range

5.5-6.7

pKa 

6.1

density

1.23 g/cm3 at 20—25 °C
1.27 g/cm3 at 20—25 °C

application(s)

clinical research
diagnostic assay manufacturing
life science and biopharma (
)
metabolomics (
)

SMILES string

OS(=O)(=O)CCN1CCOCC1

InChI

1S/C6H13NO4S/c8-12(9,10)6-3-7-1-4-11-5-2-7/h1-6H2,(H,8,9,10)

InChI key

SXGZJKUKBWWHRA-UHFFFAOYSA-N

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General description

MES Buffer, recognized as one of the "Good" buffers employed in biological and biochemical applications, plays a crucial role across various research domains. This zwitterionic and morpholinic buffer proves highly valuable due to its effective buffering capacity within the pH range of 5.5 to 6.7. Notably, MES Buffer does not engage in complex formation with the majority of metals commonly employed in environmental and biological studies. Its solubility in water is excellent, and it exhibits minimal lipid solubility, rendering it impermeable to membranes.

This versatile buffer may find widespread application in maintaining a stable environment in solutions dedicated to cell culture media, protein-based buffer formulations, and electrophoresis running buffers. Its significance extends to the purification processes of antibodies, peptides, proteins, blood components, and growth factors. MES Buffer stands out as an optimal choice for capillary electrochromatography, offering low ionic mobility. Notably, it serves as a safer alternative to potentially toxic agents like cacodylate and non-zwitterionic buffers, including citrate and malate. MES Buffer also proves valuable for fine-tuning the pH of growth media, stabilizing enzymatic solutions, and serving as a component of PAGE running buffer in diverse experimental settings.

Application

It has been utilized as a buffer bicarbonate‐containing culture medium
MES has been used as a component of the elution buffer used to elute plasma from α2-macroglobulin affinity column. It has been used as an activation buffer during antibody conjugation to microparticles. MES has been used in the functionalization of microchannels during the creation of microfluidic-integrated surface plasmon resonance (SPR) platform for pathogen detection.

Features and Benefits

  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • Effective Buffering from pH 5.5-6.7 (25 °C) with a pKa of 6.1 (25 °C)
  • Highly soluble in water
  • Minimal metal ion binding
  • Less toxic to cells than other buffers such as Tris and phosphate
  • Stable in a wide pH range
  • Low UV absorptivity and Minimal reactivity

Quality

Low moisture

Other Notes

For additional information on our range of Biochemicals, please complete this form.

comparable product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000385636
0000385637
0000386406
0000385638
0000369943

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What does low moisture content mean in the description of Product M3671, MES - low moisture?

    The amount of water/moisture for MES (M3671) is tested by Karl Fisher and meets a specification of less than 1%. The other MES Hydrates offered have a specification of less than 9%.

  4. What is the solubility of Product M3671, MES - low moisture?

    We test the solubility of Prod. No. M3671 at 20 grams in 80 mL of water. A clear, colorless solution is obtained. This concentration is not necessarily the upper solubility limit of MES in water.

  5. What is the pH of a solution of Product M3671, MES - low moisture?

    The pH of a 0.5 M solution in water should be between 2.5 and 4.0.

  6. Is Product M3671, MES - low moisture, autoclavable?

    Autoclaving is not recommended for any sulfonic acid buffer solution. Sterilization of a MES buffer solution can be accomplished by filtering it through a 0.2 micron filter.

  7. How does one prepare a buffer using Product M3671, MES - low moisture, free acid?

    A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH. Alternatively, volumes of equimolar MES free acid and sodium MES can be mixed to attain the desired pH.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Sensitive Mie scattering immunoagglutination assay of porcine reproductive and respiratory syndrome virus (PRRSV) from lung tissue samples in a microfluidic chip
Song, Jae-Young, et al.
J. Virol. Methods, 178, 31-38 (2011)
Impaired lipoprotein receptor-mediated peripheral binding of plasma amyloid-? is an early biomarker for mild cognitive impairment preceding Alzheimer's disease.
Sagare, Abhay P., et al.
Journal of Alzheimer'S Disease, 24 (1), 25-34 (2011)
Portable microfluidic integrated plasmonic platform for pathogen detection
Tokel, Onur, et al.
Scientific Reports, 2015, 9152-9152 null
Sensitive Mie scattering immunoagglutination assay of porcine reproductive and respiratory syndrome virus (PRRSV) from lung tissue samples in a microfluidic chip.
Song JY, Lee CH, Choi EJ, Kim K, et al.
J. Virol. Methods, 178, 31-38 (2011)
"(Un) suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions-a review.
Ferreira, Carlos MH, et al.
Royal Society of Chemistry Advances, 5 (39), 30989-31003 (2015)
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