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M0894

Sigma-Aldrich

Minimum Essential Medium Eagle

Alpha Modification, with L-glutamine and sodium pyruvate, without ribonucleosides, deoxyribonucleosides and sodium bicarbonate, powder, suitable for cell culture

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Synonym(s):
MEM
UNSPSC Code:
12352207
NACRES:
NA.71

Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

components

stable glutamine: no
Hanks’ salts (2% CO2): no
sodium pyruvate: yes
NaHCO3: no
phenol red: yes
glucose: yes
L-glutamine: yes
Earle’s salts (5% CO2): yes
HEPES: no

shipped in

ambient

storage temp.

2-8°C

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General description

This is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins.

Quantity

Formulated to contain 10.1 grams of powder per liter of medium.

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

动植物来源培养基

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jason W Ashley et al.
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Journal of lipid research, 61(2), 143-158 (2019-12-11)
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The periodontal ligament (PDL) is a connective tissue that attaches the tooth cementum to the alveolar bone and is derived from dental follicle cells (DFCs). The DFCs form fibroblasts, osteoblasts, cementoblasts, and PDL stem cells (PDLSCs). We previously reported homeobox
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Osteoporotic fracture is the main complication of osteoporosis (OP) and accounts for millions of injuries annually. Local intervention by intra-marrow injection has been a good option for preventing osteoporotic bone loss when the osteoporotic femoral fracture has been treated. In
Kateřina Valentová et al.
International journal of molecular sciences, 19(8) (2018-08-12)
Silymarin, an extract from milk thistle (Silybum marianum) fruits, is consumed in various food supplements. The metabolism of silymarin flavonolignans in mammals is complex, the exact structure of their metabolites still remains partly unclear and standards are not commercially available.

Articles

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Protocols

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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