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M0393

Medium 199

With Hanks′ salts and ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

M199 Medium, TCM 199

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
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Product Name

Medium 199, With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
Hanks’ salts (2% CO2): yes
sodium pyruvate: no
NaHCO3: no
L-glutamine: yes
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Application

Medium 199 has been used:
  • to test and determine the pH sensitivity of the sensors in a complex environment
  • as a culture medium during oocyte harvesting
  • as a component of Leishmania donovani (LdBOB) promastigote media for culturing L. donovani
  • to culture visceral adipose tissue explants

Preparation Note

Formulated to contain 10.6 grams of powder per liter of medium.
Supplement with 0.35 g/L sodium bicarbonate.

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Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000537607
0000503266
0000503263
0000503263
0000537607

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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

粉末培养基制备说明

粉末培养基及盐混合物极具吸湿性，应注意防潮。

Articles

Medium 199 - M199 Formulations

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Thomas H C Sit et al.
Nature, 586(7831), 776-778 (2020-05-15)
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first detected in Wuhan in December 2019 and caused coronavirus disease 2019 (COVID-19)1,2. In 2003, the closely related SARS-CoV had been detected in domestic cats and a dog3. However, little is known about the
Song Wang et al.
Frontiers in physiology, 8, 1004-1004 (2017-12-23)
P311 was identified to markedly promote cutaneous wound healing by our group. Angiogenesis plays a key role in wound healing. In this study, we sought to define the role of P311 in skin wound angiogenesis. It was noted that P311
Anna Gioseffi et al.
Life science alliance, 3(12) (2020-10-31)
Leishmania donovani infection of macrophages results in quantitative and qualitative changes in the protein profile of extracellular vesicles (EVs) released by the infected host cells. We confirmed mass spectrometry results orthogonally by performing Western blots for several Leishmania-infected macrophage-enriched EVs
View All Related Papers

Global Trade Item Number

SKUGTIN
M0393-10X1L04061835518982
M0393-50L04061833993101
M0393-25KG04061834408833