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Key Documents

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Safety Information

L7533

Sigma-Aldrich

LB Broth with agar (Lennox)

EZMix powder microbial growth medium

Synonym(s):

LB broth

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¥3,652.98

¥3,652.98

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96 TESTS
¥3,652.98

About This Item

UNSPSC Code:
41106200
NACRES:
NA.85

¥3,652.98

Please contact Customer Service for Availability

grade

Molecular Biology

Quality Level

200

sterility

non-sterile

form

powder

composition

Agar, 15 g/L
NaCl, 5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

technique(s)

microbiological culture: suitable

application(s)

agriculture
food and beverages
microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

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SRP3084SRP3327SRP3320
IL-1RA murine recombinant, expressed in E. coli, ≥98% (SDS-PAGE)

SRP6006

IL-1RA murine

IL-1Ra human recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

SRP3084

IL-1Ra human

IL-1 RECEPTOR ANTAGONIST human Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

SRP3327

IL-1 RECEPTOR ANTAGONIST human

IL-36RA human recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

SRP3320

IL-36RA human

assay

≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

biological source

mouse

biological source

human

biological source

human

biological source

human

mol wt

17.4 kDa

mol wt

17.2 kDa

mol wt

17.2 kDa

mol wt

17.0 kDa

form

lyophilized

form

lyophilized

form

lyophilized

form

lyophilized

General description

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

Application

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

Features and Benefits

Lennox EZMix powder with agar provides:
  • A budget-friendly alternative to pre-poured plates
  • Granulated, dust-free format for safer handling and faster mixing
  • Convenient small package to eliminate weighing
  • Easy scale-up using larger package sizes
  • Standard formulation

Preparation Note

1. Suspend 35.6 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.
To prepare Lennox L Agar: Add 1 g glucose and proceed with preparation instructions as above.
To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1 M magnesium sulfate after autoclaving.

Reconstitution

Add 35.6g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired). Pour into petri dishes and allow to solidify.

Legal Information

Zeocin is a registered trademark of Cayla Sarl

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Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

新产品

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    0000392099
    0000392100
    0000368716
    0000288422
    0000304826

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    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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    Find documentation for the products that you have recently purchased in the Document Library.

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    1. Which document(s) contains shelf-life or expiration date information for a given product?

      If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    2. How do I get lot-specific information or a Certificate of Analysis?

      The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    3. How do I find price and availability?

      There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

    4. What is the Department of Transportation shipping information for this product?

      Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

    5. What are the differences among the Luria, Lennox and Miller LB formulations?

      LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose.  Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version).  For some applications, even lower salt is required (Luria's low salt version). 

    6. What is the difference among the many LB - Lennox products?

      LB - Lennox is available in many types to suit your needs.  The different product formats include powder, EZMix™ powder, and tablet form.  Each of these forms are available with and without agar for easy LB-agar plate preparation.L3022 (LB Lennox powder)L2897 (LB Lennox powder with agar)L7275 (LB Lennox tablets)L7025 (LB Lennox tablets with agar)L7658 (LB Lennox EZMix(TM) powder)L7533 (LB Lennox EZMix(TM) powder with agar)L5542 (LB Lennox pre-poured agar plates)

    7. Which bacterial culture medium is the best choice for my application?

      Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production.  The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.

    8. Will adding magnesium to the culture medium increase cell density?

      In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities.  You may need to also increase the shaking speed of the incubator. 

    9. My question is not addressed here, how can I contact Technical Service for assistance?

      Ask a Scientist here.

    Sambrook, J., et al
    Molecular Cloning: A Laboratory Manual, 2, 1-1 (1989)
    Ausubel, F. M.
    Current Protocols in Molecular Biology, 1, 3-3 (1994)
    Alexis Kaushansky et al.
    Nature protocols, 5(4), 773-790 (2010-04-03)
    Protein microarrays provide an efficient way to identify and quantify protein-protein interactions in high throughput. One drawback of this technique is that proteins show a broad range of physicochemical properties and are often difficult to produce recombinantly. To circumvent these
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    Quiescence is a fundamental property that maintains hematopoietic stem cell (HSC) potency throughout life. Quiescent HSCs are thought to rely on glycolysis for their energy, but the overall metabolic properties of HSCs remain elusive. Using combined approaches, including single-cell RNA

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    General protocols for growth of competent cells and their transformation (uptake of DNA).

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    Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.

    Microbial Growth Protocols

    General protocols for growth of competent cells in microbial medium.

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