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L7391

Sigma-Aldrich

Anti-LIS1 antibody, Mouse monoclonal

clone LIS1-338, purified from hybridoma cell culture

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MDL number:
MFCD03457563
UNSPSC Code:
12352203
PubChem Substance ID:
24896475
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

LIS1-338, monoclonal

form

buffered aqueous solution

mol wt

antigen 46 kDa

species reactivity

zebrafish, chicken, rat, human, bovine, mouse

concentration

~2 mg/mL

technique(s)

immunoprecipitation (IP): suitable
microarray: suitable
western blot: 0.5-1 μg/mL using a rat brain cytosol preparation

isotype

IgG1

UniProt accession no.

P43034

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

SMILES string

CCCCC\C=C\C\C=C\CCCCCCCC(=O)OCC(O)COP(O)(O)=O

InChI

1S/C21H39O7P/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-21(23)27-18-20(22)19-28-29(24,25)26/h6-7,9-10,20,22H,2-5,8,11-19H2,1H3,(H2,24,25,26)/b7-6+,10-9+

InChI key

ZQTAMPRZFOOEEP-AVQMFFATSA-N

Gene Information

human ... PAFAH1B1(5048)
mouse ... Pafah1b1(18472)
rat ... Pafah1b1(83572)

General description

Monoclonal Anti-LIS1 (mouse IgG1 isotype) is derived from the LIS1-338 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a recombinant human LIS1 protein. The LIS1 protein (46 kDa) contains seven WD (tryptophan-aspartic acid) repeats, a motif shared by at least 140 known proteins.
The gene PAFAH1B1 (platelet activating factor acetylhydrolase 1b regulatory subunit 1) encodes a protein named LIS1 (lissencephaly-1) that forms the α subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase (PAFAH). The gene is mapped to human chromosome 17p13.3.

Specificity

By immunoblotting, the antibody prefers the phosphorylated form of the LIS1 molecule (46 kDa).

Immunogen

recombinant human LIS1 protein.

Application

Monoclonal Anti-LIS1 antibody produced in mouse has been used in protein pulldown assay and immunofluorescence.

Biochem/physiol Actions

LIS1 is involved in cell regulation, including the β subunits of G-proteins (Gβ). This protein motif is likely to mediate protein-protein interactions. LIS1 is a protein that is highly conserved during evolution; bovine, mouse, and chicken proteins exceed 99% similarity.
The heterotrimeric enzyme, platelet-activating factor acteylhydrolase (PAFAH), catalyzes the cleavage of acetyl group at the SN-2 position of platelet-activating factor. The α subunit encoded by the gene PAFAH1B1 (platelet activating factor acetylhydrolase 1b regulatory subunit 1) interacts with tubulin and affects microtubule dynamics. Mutations in this gene have been linked to Miller–Dieker lissencephaly, a human brain malformation characterized by a smooth cerebral surface and a disordered organization of the cortical layers resulting from a defect in neuronal migration.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Interaction between LIS1 and doublecortin, two lissencephaly gene products
Caspi M, et al.
Human Molecular Genetics, 9(15), 2205-2213 (2000)
Shusheng Wang et al.
The Journal of biological chemistry, 286(1), 587-593 (2010-11-09)
The nuclear distribution protein E (NudE) and nuclear distribution protein E-like (Nudel or Ndel1) interact with both lissencephaly 1 (Lis1) and dynein. These interactions are thought to be essential for dynein function. Previous studies have shown that the highly conserved
Liansheng Liu et al.
Cell reports, 22(12), 3277-3291 (2018-03-22)
Intracellular transport of membranous organelles and protein complexes to various destinations is fundamental to signaling transduction and cellular function. The cytoplasmic dynein motor and its regulatory proteins LIS1 and NDE1 are required for transporting a variety of cellular cargos along
Richard J McKenney et al.
The Journal of biological chemistry, 286(45), 39615-39622 (2011-09-14)
Cytoplasmic dynein is responsible for a wide range of cellular roles. How this single motor protein performs so many functions has remained a major outstanding question for many years. Part of the answer is thought to lie in the diversity
Armen J Moughamian et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(32), 13190-13203 (2013-08-09)
Long-range retrograde axonal transport in neurons is driven exclusively by the microtubule motor cytoplasmic dynein. The efficient initiation of dynein-mediated transport from the distal axon is critical for normal neuronal function, and neurodegenerative disease-associated mutations have been shown to specifically
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