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L3544

Sigma-Aldrich

Anti-Lamin A−Atto 647N antibody produced in rabbit

1.5-3 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-LAMA, Anti-LMNA, Anti-LNMI, Anti-Lamin A/C

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About This Item

MDL number:
MFCD01633588
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

Atto 647N conjugate

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

concentration

1.5-3 mg/mL

technique(s)

direct immunofluorescence: 5-10 μg/mL using human HeLa cells

UniProt accession no.

P02545

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... LMNA(4000)

General description

Lamin A is a structural protein of the nuclear lamina. The nuclear lamina is a meshwork of intermediate filaments that underlies the inner face of the nuclear envelope. The precursor of lamin A, prelamin comprises 98 unique amino acids and also harbors the farnesyl group in C-terminus post-synthesis. The cleavage of the farnesyl group containing end 18 amino acids results in mature Lamin A. Lamin A is mapped to human chromosome 1q21.2.

Specificity

Anti-Lamin A-Atto 647N recognizes human Lamin A.

Application

Anti-Lamin A−Atto 647N antibody produced in rabbit may be used in direct immunofluorescence.

Biochem/physiol Actions

Lamin A is cleaved into a 47 kDa fragment during apoptosis. Lamin A cleavage seems to be essential for chromatin condensation and nuclear disassembly in apoptosis. Mutations in Lamin A and C have been linked to a variety of rare human diseases including muscular dystrophy, lipodystrophy, cardiomyopathy, neuropathy and progeroid syndromes (collectively termed laminopathies) and to premature aging (Hutchinson-Gilford progeria syndrome).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Protect from prolonged exposure to light. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Certificates of Analysis (COA)

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The nuclear lamina and inherited disease
Worman HJ and Courvalin JC
Trends in Cell Biology, 12(12), 591-598 (2002)
The lamin protein family
Dittmer TA and Misteli T
Genome Biology, 12(5), 1-14 (2011)
Ning-Ang Liu et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 29(6), 2514-2525 (2015-03-04)
DNA double-strand breaks (DSBs) are the major lethal lesion induced by ionizing radiation (IR). RAD51-dependent homologous recombination (HR) is one of the most important pathways in DSB repair and genome integrity maintenance. However, the mechanism of HR regulation by RAD51

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