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Merck
CN

L2025

Luria Agar Base, Miller

Tryptone, yeast extract, sodium chloride and agar

Synonym(s):

LA, modified, Luria-Bertani media, modified, Lysogeny broth, modified

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About This Item

UNSPSC Code:
41106200
NACRES:
NA.72
eCl@ss:
42040102
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form

powder

technique(s)

microbiological culture: suitable

application(s)

agriculture
microbiology

Quality Level

General description

Luria Bertani (LB) medium is one of the complex media that is widely used to grow Escherichia coli in small-scale cultures.

Application

Luria Agar Base, Miller has been used as a growth medium during plasmid amplification. It has also been used as a culture medium for strains of Penicillium sp. laika.
Luria broth/agar (LA), Miller (L1900) is a bacteria growth mixture of sodium chloride (0.5g/L); tryptone (10g/L) yeast extract (5g/L) and agar (15g/L) used to culture members of the Enterobacteriaceae family and for coliphage plaque assays.

Preparation Note

Recommended use at 30.5 g per liter.

Other Notes

Contains: Tryptone, yeast extract, sodium chloride and agar.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Chujin Ruan et al.
Current biology : CB, 32(24), 5285-5294 (2022-12-02)
The amount of bacterial diversity present on many surfaces is enormous; however, how these levels of diversity persist in the face of the purifying processes that occur as bacterial communities expand across space (referred to here as range expansion) remains
Daria Julkowska et al.
Journal of bacteriology, 187(1), 65-76 (2004-12-17)
The natural wild-type Bacillus subtilis strain 3610 swarms rapidly on the synthetic B medium in symmetrical concentric waves of branched dendritic patterns. In a comparison of the behavior of the laboratory strain 168 (trp) on different media with that of
Anthony S Danko et al.
Applied and environmental microbiology, 70(10), 6092-6097 (2004-10-07)
Pseudomonas putida strain AJ and Ochrobactrum strain TD were isolated from hazardous waste sites based on their ability to use vinyl chloride (VC) as the sole source of carbon and energy under aerobic conditions. Strains AJ and TD also use
Mario Losen et al.
Biotechnology progress, 20(4), 1062-1068 (2004-08-07)
Shake-flask cultures are widely used for screening of high producing strains. To select suitable strains for production scale, cultivation parameters should be applied that provide optimal growth conditions. A novel method of measuring respiratory activity in shake-flask cultures was employed
Yu-Fan Chuang et al.
Frontiers in cell and developmental biology, 9, 667879-667879 (2021-06-29)
Specific changes in the genome have been accomplished by the revolutionary gene-editing tool known as clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system. The advent of programmable RNA editing CRISPR/Cas nucleases has made this gene-editing tool safer and more

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