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K3753

Sigma-Aldrich

Krebs-Henseleit Buffer Modified

With 2000 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture

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1 KG
CN¥1,198.93
3 X 1 KG
CN¥3,027.27
5 KG
CN¥3,852.17
12 KG
CN¥9,202.72
25 KG
CN¥12,080.83

CN¥1,198.93

Estimated to ship onApril 11, 2025Details

Request a Bulk Order
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1 KG
CN¥1,198.93
3 X 1 KG
CN¥3,027.27
5 KG
CN¥3,852.17
12 KG
CN¥9,202.72
25 KG
CN¥12,080.83

About This Item

UNSPSC Code:
12352207

CN¥1,198.93

Estimated to ship onApril 11, 2025Details

Request a Bulk Order

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

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NA0150NA0200NA0150S
GenElute™ HP Plasmid Miniprep Kit sufficient for 350 preparations

NA0160

GenElute™ HP Plasmid Miniprep Kit

GenElute™ HP Plasmid Miniprep Kit sufficient for 70 preparations

NA0150

GenElute™ HP Plasmid Miniprep Kit

GenElute™ HP Plasmid Midiprep Kit sufficient for 25 purifications

NA0200

GenElute™ HP Plasmid Midiprep Kit

GenElute™ HP Plasmid Miniprep Kit sufficient for 10 preparations

NA0150S

GenElute™ HP Plasmid Miniprep Kit

usage

sufficient for 350 preparations

usage

sufficient for 70 preparations

usage

sufficient for 25 purifications

usage

sufficient for 10 preparations

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

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sustainability

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sustainability

Greener Alternative Product

sustainability

Greener Alternative Product

sustainability

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NA0150

NA0150S, NA0160, NA9604

NA0200S

NA0150, NA0160, NA9604

greener alternative category

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greener alternative category

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greener alternative category

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General description

Krebs-Henseleit Buffer Modified is a balanced salt solution (BSS) that is used in cell culture and plays a role in providing cells with water and inorganic salts for normal cell metabolism. In addition, it provides a buffering system to maintain the optimal pH of the medium and acts as an irrigating, transporting, and diluting agent to maintain the intra- and extra-cellular osmotic balance. BSS provides glucose as an energy source for cell metabolism.

Application

Krebs-Henseleit Buffer Modified has been used:

  • to collect the rat brains for ex vivo brain slice experiments[1]
  • to measure calcium and sodium in rat pulmonary arterial endothelial cells (PAECs)[2]
  • to collect the uterine and forelimb blood vessel samples from horses[3]

Quantity

Formulated to contain 9.6 grams of powder per liter of medium.

Reconstitution

Supplement with 2.1 g/L sodium bicarbonate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000378773
    0000315222
    0000376329
    0000302561
    0000302559

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    Protocols

    Powdered Media Preparation Instructions

    Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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