Key Documents
Safety Information
HWGCRISPR
Sigma Whole Human Genome Lentiviral CRISPR Pool
About This Item
Recommended Products
packaging
pkg of 8x25 μL (vials)
Quality Level
concentration
5x108 VP/ml (via p24 assay)
application(s)
CRISPR
shipped in
dry ice
storage temp.
−70°C
1 of 4
This Item | RAB0322 | RAB0250 | RAB0305 |
|---|---|---|---|
| Gene Information human ... IL10RB(3588) | Gene Information human ... IL9(3578) | Gene Information human ... IL11(3589) | Gene Information human ... IL5RA(3568) |
| species reactivity human | species reactivity human | species reactivity human | species reactivity human |
| assay range inter-assay cv: <12% | assay range inter-assay cv: <12% | assay range inter-assay cv: <12% | assay range inter-assay cv: <12% |
| input sample type cell culture supernatant(s) | input sample type plasma | input sample type plasma | input sample type cell culture supernatant(s) |
| technique(s) ELISA: suitable, capture ELISA: suitable | technique(s) ELISA: suitable, capture ELISA: suitable | technique(s) ELISA: suitable, capture ELISA: suitable | technique(s) ELISA: suitable, capture ELISA: suitable |
| detection method colorimetric | detection method colorimetric | detection method colorimetric | detection method colorimetric |
General description
Application
Biochem/physiol Actions
Features and Benefits
- Use CRISPR nucleases to knockout protein-coding genes to assess their function
- Efficiently screen the whole human genome (16,000+ genes) at the bench-top without robotics or specialized equipment
- Increased flexibility consisting of 8 subpools at 23,000 clones per pool (184,000 clones total), from the previous 2 subpools (Gecko v2)
- Expanded gRNA coverage at 10 gRNAs per gene, from the previous 6 gRNAs per gene (Gecko v2)
- Numerous built-in enrichment and depletion controls allows researchers to confidently gauge the success of their pooled screening experiments
- 2 Vector System (pools are gRNA-only, Cas9 sold separately)
- Ease of optimization: Utilizes same vector system as Gecko v2 allowing for optimization one for both systems
- Minimize off-targeting: stringent gRNA design and tiling rules
Preparation Note
Prior to performing a library-scale screening, two preliminary experiments must be conducted: (1) determine the sensitivity of target cell type to puromycin (kill curve), and (2) determine the functional titer of the lentivirus in your cell type by completing a colony-forming assay (measured in CFU/ml). The calculation of MOI (multiplicity of infection) should be based on the value of CFU. Different cell types vary in transduction efficiency and different lentiviral constructs do not behave identically, so it is critical to optimize your experimental conditions with control lentiviral CRISPR clones (available from Sigma) prior to performing your pooled experiment.
Other Notes
Legal Information
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
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Articles
Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.
Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.
Protocols
Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.
FACS sorts cells based on light scattering and fluorescence for objective cell analysis.
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