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H9661

Hepatocyte Growth Factor human

≥95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

Synonym(s):

HGF, Hepatopoietin A, Scatter Factor

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77
MDL number:
MFCD00217422

Key Documents

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Product Name

Hepatocyte Growth Factor human, HGF, recombinant, expressed in NSO cells, suitable for cell culture

biological source

human

recombinant

expressed in NSO cells

assay

≥95% (SDS-PAGE)

form

lyophilized powder

potency

20-40 ng/mL

quality

endotoxin tested

mol wt

70-80 kDa by SDS-PAGE (non-reducing)

packaging

pkg of 5 and 25 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤4 ng/mL (ED50)

UniProt accession no.

P14210

storage temp.

−20°C

Quality Level

200

Gene Information

human ... HGF(3082)

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Analysis Note

The biological activity is measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells.

Application

Hepatocyte Growth Factor human has been used as a growth factor cocktail to suspend rat fibrogen/thrombin cells. It has also been used to study the tyrosine phosphorylation of MET (MET proto-oncogene, receptor tyrosine kinase) in myxofibrosarcoma cell line.

Biochem/physiol Actions

HGF′s major function is to induce in vitro hepatocytes proliferation. HGF is also essential for cell migration and angiogenesis. Itis associated with embryonic development and tissue homeostasis. It is known to mediate placental trophoblast invasion, which is essential for normal fetal growth. Abnormal and defective HGF causes preeclampsia due to placental insufficiency. HGF exhibits its multifunctions through c-MET (proto-oncogene tyrosine kinase receptor). HGF is known to induce human umbilical vein endothelial cell proliferation and vasculogenic mimicry migration in gastric cancer cells, thus serving as a good target for cancer therapy. Upregulation of the HGF gene is observed in advanced stages of cancer, such as pancreatic adenocarcinoma.
Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of cell types including endothelial and epithelial cells, melanocytes, and keratinocytes. It also mediates epithelial morphogenesis. HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene.

General description

Recombinant, human hepatocyte growth factor (HGF) is produced from the 728 amino acid residue variant of the human pre-pro-HGF protein 6 and expressed in a mouse myeloma cell line NSO.The HGF gene is mapped to human chromosome 7q21.1. The encoded protein is a mesenchymal-fibroblast derived cytokine.

Other Notes

The NSO-expressed recombinant HGF is greater than 95% heterodimeric HGF. The insect cell line-expressed recombinant HGF is a mixture of single chain HGF (predominant) with some heterodimeric HGF. Single chain HGF and heterodimeric HGF are equally active in in vitro assays.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 50 μg of bovine serum albumin per 1μg of cytokine.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
0000496772
0000496772
0000479155
0000479155
0000460763

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Correlation between HGF/c-Met and Notch1 signaling pathways in human gastric cancer cells
Huang KH, et al.
Oncology Reports, 40(1), 294-302 (2018)
Dual inhibition of Hedgehog and c-Met pathways for pancreatic cancer treatment
Rucki AA, et al.
Molecular Cancer Therapeutics (2017)
Negative control of the HGF/c-MET pathway by TGF-beta: a new look at the regulation of stemness in glioblastoma
Papa E, et al.
Cell Death & Disease, 8(12), 3210-3210 (2017)
Analysis of hepatocyte growth factor immunostaining in the placenta of HIV-infected normotensive versus preeclamptic pregnant women
Cele SB,et al.
Eur. J. Obstet. Gyn. Rep. Biol. (2018)
HGF derived from cancer-associated fibroblasts promotes vascularization in gastric cancer via PI3K/AKT and ERK1/2 signaling
Ding X, et al.
Oncology Reports (2018)
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Hepatocyte Growth Factor (HGF)

Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of other cells, including endothelial and epithelial cells, melanocytes, and keratinocytes.

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Related Content

Data Sheet - H9661

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