H7166
Interleukin-3 human
IL-3, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture
Synonym(s):
IL-3
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About This Item
biological source
human
Quality Level
recombinant
expressed in HEK 293 cells
Assay
>95% (SDS-PAGE)
form
lyophilized powder
potency
≤2 ng/mL EC50
quality
endotoxin tested
mol wt
dimer 17-45 kDa (glycosylated)
packaging
pkg of 10 μg
manufacturer/tradename
HumanZyme
technique(s)
cell culture | mammalian: suitable
impurities
<1 EU/μg
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... IL3(3562)
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Biochem/physiol Actions
Interleukin-3 (IL-3) is a multifunctional protein, originally called colony forming unit-stimulating activity (CFU-SA), and is produced by activated T lymphocytes. IL-3 supports the formation of multilineage colonies in the early development of multipotent hematopoietic progenitor cells. IL-3 has been shown to induce colony formation of macrophages, neutrophils, mast cells, and megakaryocytes from agar-suspended bone marrow cells. IL-3 also interacts with IL-2 to stimulate growth of T lymphocytes and to induce IgG secretion from activated B cells. Other synonyms for IL-3 include pan-specific hemopoietin, multicolony stimulating factor, mast cell growth factor, burst promoting activity, histamine-producing cell stimulating factor, P cell stimulating factor, and WEHI-3 factor.
Physical form
Lyophilized from a 0.2 μm filtered solution of 1x PBS.
Preparation Note
HumanKine Interleukin-3 (IL-3), expressed in human HEK 293 cells, is a glycosylated monomer with an apparent molecular mass of 17-45 kDa due to glycosylation, which is absent when this cytokine is expressed in E. coli. Production in human HEK 293 cells offers authentic glycosylation which contributes to stability in cell growth media and other applications.
Analysis Note
The activity was determined by the dosedependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
Legal Information
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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