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H3784

Sigma-Aldrich

HEPES sodium salt

BioPerformance Certified, suitable for cell culture, ≥99.0%

Synonym(s):

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt

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25 G
CN¥584.64
100 G
CN¥2,325.78
500 G
CN¥8,599.99
1 KG
CN¥14,419.49

CN¥584.64

Available to ship onApril 27, 2025Details

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25 G
CN¥584.64
100 G
CN¥2,325.78
500 G
CN¥8,599.99
1 KG
CN¥14,419.49

About This Item

Empirical Formula (Hill Notation):
C8H17N2NaO4S
CAS Number:
75277-39-3
Molecular Weight:
260.29
EC Number:
278-169-7
MDL number:
MFCD00036463
UNSPSC Code:
12161700
PubChem Substance ID:
24895595
NACRES:
NA.25

CN¥584.64

Available to ship onApril 27, 2025Details

Request a Bulk Order

grade

BioPerformance Certified

Quality Level

400

Assay

≥99.0%

form

powder

storage condition

dry at room temperature

technique(s)

activity assay: suitable
cell culture | mammalian: suitable

impurities

endotoxin and total aerobic microbial count, tested

color

white

pH

10-12 (1 M in water)

useful pH range

6.8-8.2

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H7006H3662H8651
HEPES sodium salt BioPerformance Certified, suitable for cell culture, ≥99.0%

H3784

HEPES sodium salt

HEPES sodium salt ≥99.0% (titration)

H7006

HEPES sodium salt

HEPES sodium salt solution BioReagent, 1M, suitable for cell culture

H3662

HEPES sodium salt solution

HEPES sodium salt BioXtra, ≥99.0% (titration)

H8651

HEPES sodium salt

assay

≥99.0%

assay

≥99.0% (titration)

assay

-

assay

≥99.0% (titration)

grade

BioPerformance Certified

grade

-

grade

-

grade

-

technique(s)

activity assay: suitable, cell culture | mammalian: suitable

technique(s)

HPLC: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

Quality Level

400

Quality Level

400

Quality Level

200

Quality Level

-

form

powder

form

powder

form

solution

form

powder

application(s)

cell analysis
diagnostic assay manufacturing
general analytical
life science and biopharma

application(s)

diagnostic assay manufacturing
general analytical (
)
life science and biopharma
metabolomics

application(s)

-

application(s)

diagnostic assay manufacturing

General description

HEPES, or 4-(2-hydroxyethyl)piperazine-1-ethane-sulfonic acid, is a zwitterionic N-substituted aminosulfonic acid buffer with a remarkable pH buffering capacity between 6.8 and 8.2 (pKa of 7.48 at 25°C). Widely recognized for its versatility, HEPES is extensively employed in cell biology, biochemical, and biological research. Notably, its neutrality towards metal ions makes it a preferred choice for solutions with metal ions, acting as a "non-coordinating buffer."

In cell culture, HEPES excels in maintaining stable physiological pH even amidst fluctuating carbon dioxide concentrations, outperforming bicarbonate buffers (NaHCO3). Moreover, HEPES showcases valuable properties in various biological and biochemical processes, such as isoelectric focusing for protein separation and minimal interference in DNA-restriction enzyme reactions, making it indispensable across diverse research applications, including immunoprecipitation, cell lysis, and live cell imaging. Its versatility, exceptional buffering capacity, and minimal interaction with molecules establish HEPES as a key tool in various research domains.

Application

HEPES sodium salt has been used:
  • to supplement the HSOF medium[1]
  • as a buffer component of HCZB medium in egg and embryo culture[2]
  • as a buffer component in cell culture[3]

Features and Benefits

  • High-purity product for biochemical and biological research
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • Highly soluble in water with a useful pH range of 6.8 - 8.2 and pKa of 7.5 at 25 °C
  • Suitable for Cell culture
  • Tested for Endotoxins and Total Aerobic Microbial Count
  • Free from DNase, NICKase, RNase, Endonuclease, Exonuclease and Protease

Physical form

Lyophilized, spray dried, or crystallized material.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000435518
    0000435516
    0000435516
    0000435518
    0000430362

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    Cell reports, 31(5), 107611-107611 (2020-05-07)
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    Nature biotechnology, 38(3), 297-302 (2020-02-26)
    The scarcity of donor organs may be addressed in the future by using pigs to grow humanized organs with lower potential for immunological rejection after transplantation in humans. Previous studies have demonstrated that interspecies complementation of rodent blastocysts lacking a
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