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HEPES sodium salt

EMPROVE® EVOLVE

Pharma Manufacturing

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Synonym(s):
HEPES, Sodium Salt, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt
Empirical Formula (Hill Notation):
C8H17N2NaO4S
CAS Number:
75277-39-3
Molecular Weight:
260.29
MDL number:
MFCD00036463
UNSPSC Code:
12161700

biological source

synthetic

Quality Level

400

product line

EMPROVE® EVOLVE

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, microbial, and trace metals; tested

pH

6.8-8.2

useful pH range

6.8-8.2

pKa (25 °C)

7.5

pKa 

7.5

suitability

suitable for manufacturing use

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

storage temp.

2-30°C

SMILES string

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

InChI key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

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Application

HEPES Sodium is a biological buffer often referred to as a “Good′s” buffer. HEPES Sodium, used in conjunction with HEPES, is the most commonly used biological buffer formulation since the pKa of the buffer is 7.31 which corresponds to the physiological pH of 7.36. HEPES Sodium is considered to be non-toxic to culture cell lines, highly water soluble and provides high-solution clarity.HEPES Sodium is used in cell culture media, biopharmaceutical buffer formulations (both upstream and downstream) and diagnostic reagent formulations.

Legal Information

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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