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H3757

Sigma-Aldrich

Hyaluronidase from bovine testes

Type VIII, lyophilized powder, 300-1,000 U/mg

Synonym(s):

Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase

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About This Item

CAS Number:
37326-33-3
Enzyme Commission number:
3.2.1.35 (BRENDA, IUBMB)
EC Number:
253-464-3
MDL number:
MFCD00131351
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bovine testis

Quality Level

200

conjugate

conjugate (Glucosaminoglycan)

type

Type VIII

form

lyophilized powder

specific activity

300-1,000 U/mg

mol wt

~55 kDa (four subunits of 14 kDa each)

UniProt accession no.

Q5E985
Q8SQG8

relevant disease(s)

cancer

storage temp.

−20°C

Gene Information

cow ... HYAL1(515397) , HYAL2(281838)

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Biochem/physiol Actions

Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.
These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Unit Definition

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

Preparation Note

Prepared from aseptic filled solution of Type I-S.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

动植物源性产品

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Certificates of Analysis (COA)

Lot/Batch Number
0000411531
0000411531
0000376213
0000297333
0000379735

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How is product H3757, Hyaluronidase from bovine testes, solubilized?

    Product H3757, Hyaluronidase from bovine testes, may be reconstituted at 1 mg/mL in 0.02 M sodium phosphate buffer, pH 7.0 containing 77mM sodium chloride and 0.01% bovine serum albumin.

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Histochemistry and cell biology, 133(1), 125-134 (2009-10-16)
Membranous epithelial cells (M cells) of the follicle-associated epithelium in Peyer's patches have a high capacity for transcytosis of several viruses and microorganisms. Here, we report that we have successfully established a bovine intestinal epithelial cell line (BIE cells) and
Chaemoon Lim et al.
Journal of cancer prevention, 27(2), 122-128 (2022-07-23)
Osteosarcoma is the most frequent primary malignant bone tumor with higher incidences in children and adolescents. Despite clinical evolutions, patients with osteosacoma have had a poor prognosis. There has been increasing evidence that cancer is a stem cell disease. This
Takayuki Yamochi et al.
Journal of assisted reproduction and genetics, 38(10), 2591-2599 (2021-05-11)
To enhance the in vitro growth of porcine oocytes, we studied the effect of mural granulosa cells (MGCs) on the viability of oocytes attached to granulosa cells (oocyte-granulosa cell complexes, OGCs) that were obtained from early antral follicles. When OGCs
C Rollo et al.
Reproduction (Cambridge, England), 154(4), 375-385 (2017-09-08)
Acetylation of histone proteins is a major determinant of chromatin structure and function. Fertilisation triggers a round of chromatin remodelling that prepares the genome for the first round of transcription from the new embryonic genome. In this study we confirm
Lauren E Hamilton et al.
International journal of molecular sciences, 22(15) (2021-08-08)
The perinuclear theca (PT) of the eutherian sperm head is a cytoskeletal-like structure that houses proteins involved in important cellular processes during spermiogenesis and fertilization. Building upon our novel discovery of non-nuclear histones in the bovine PT, we sought to
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Uncover more about glycosaminoglycans and proteoglycans including the structure of glycosaminoglycans (GAGs), the different types of GAGs, and their functions.

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

Protocols

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

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