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H1136

Sigma-Aldrich

Hyaluronidase from Streptomyces hyalurolyticus

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Synonym(s):
Hyaluronate Lyase from Streptomyces hyalurolyticus
CAS Number:
37259-53-3
Enzyme Commission number:
4.2.2.1 (BRENDA, IUBMB)
EC Number:
253-430-8
MDL number:
MFCD00131347
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Streptomyces sp. (S. hyalurolyticus)

Quality Level

200

conjugate

conjugate (Glucosaminoglycan)

form

film (Clear)

packaging

ampule of 300 ×  units

storage temp.

−20°C

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General description

Hyaluronidase is widely present in nature. In humans, it is present in a number of organs and body fluids. Hyaluronidase is found in the secretions of virus, bacteriophage, fungi and bacteria. It is also present in nematode and leeches secretions. Snakes and scorpions produce hyaluronidase in their venom. Hyaluronidase is also secreted by bees, spiders, wasps, hornets, caterpillars, fishes and lizards.

Application

Hyaluronidase from Streptomyces hyalurolyticus has been used:
  • in the preparation of DMEM/F-12 (Dulbecco′s modified eagle medium/nutrient mixture F-12) media for the isolation and purification of single cells from dissociated tumour tissue
  • as a component of digestion solution for the derivation of germline stem cells from testicular tissue

Biochem/physiol Actions

Hyaluronate lyase cleaves hyaluronic acid at the β-D-GlcNAc-(1→4)-β-D-GlcA bond, yielding 3-(4-deoxy-β-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine tetra- and hexasaccharides. Unlike other hyaluronidases, this enzyme is specific for hyaluronic acid and is inactive with chondroitin and chondroitin sulfate.
Hyaluronidase is an endoglycosidase and performs its function along with exoglycosidases, like β-glucuronidase and β-N-acetyl glucosaminidase. Hyaluronidase regulates the hyaluronic acid turnover.

Unit Definition

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How should Product H1136, Hyaluronidase, be reconstituted?

    Prior to determining the activity of this enzyme, we reconstitute it in 20 mM sodium phosphate buffer, with 77 mM sodium chloride, 0.01% BSA, pH 7.0.

  4. What amount of product is present in the vial of Product H1136, Hyaluronidase?

    The dry weight of the product is not determined. The vial should contain a minimum of 300 units of enzyme.

  5. What is the molecular weight of Product H1136, Hyaluronidase?

    The molecular weight for hyaluronidase from Streptomyces hyalurolyticus has been reported to be 91,000 DALTONS as determined by gel filtration.

  6. Are any other components present in Product H1136, Hyaluronidase, besides the enzyme?

    No protein or BSA has been added as a stabilizer. The enzyme was lyophilized from 0.5 mM Tris buffer, pH 7.0.

  7. How is the activity of Product H1136, Hyaluronidase,determined?

    Sigma assays the enzyme against a USP hyaluronidase reference standard as described in USP XXII-NF XVII, 644 (1990).

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

  11. I can’t see any material in the container. Is material present in the container?

    The product is lyophilized directly into the ampule and its appearance is a clear, thin film.

Crystal structure of hyaluronidase, a major allergen of bee venom
Markovic H, et al.
Structure, 8(10), 1025-1035 (2000)
Connor C Gains et al.
Frontiers in bioengineering and biotechnology, 8, 700-700 (2020-08-09)
The Achilles tendon (AT) is comprised of three distinct sub-tendons bound together by the inter-subtendon matrix (ISTM). The interactions between sub-tendons will have important implications for AT function. The aim of this study was to investigate the extent to which
Tímea Váradi et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 60(8), 567-575 (2012-05-09)
Although trastuzumab is an efficient drug, primary and acquired resistance is a challenging problem. The authors have previously shown in mouse xenograft experiments that masking ErbB2 by hyaluronan leads to diminished binding of the antibody and consequent resistance. In the
Wen Min Lau et al.
Cancer research, 74(9), 2630-2641 (2014-03-13)
The surface marker CD44 has been identified as one of several markers associated with cancer stem cells (CSC) in solid tumors, but its ubiquitous expression in many cell types, including hematopoietic cells, has hindered its use in targeting CSCs. In
CD44v8-10 is a cancer-specific marker for gastric cancer stem cells
Lau WM, et al.
Cancer Research, canres-ca2309 (2014)
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Protocols

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

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