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Safety Information

H0537

Millipore

HIS-Select® HF Nickel Affinity Gel

Synonym(s):

Ni-NTA HF resin

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1 ML
CN¥846.49
10 ML
CN¥4,233.78
25 ML
CN¥5,406.04
100 ML
CN¥17,320.49
500 ML
CN¥70,141.54

CN¥846.49

Please contact Customer Service for Availability
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1 ML
CN¥846.49
10 ML
CN¥4,233.78
25 ML
CN¥5,406.04
100 ML
CN¥17,320.49
500 ML
CN¥70,141.54

About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

CN¥846.49

Please contact Customer Service for Availability

form

suspension

Quality Level

200

technique(s)

affinity chromatography: suitable

matrix

Highly cross-linked 6% Beaded Agarose

capacity

15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)

storage temp.

2-8°C

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This Item
P6611H8162P9740
HIS-Select® HF Nickel Affinity Gel

H0537

HIS-Select® HF Nickel Affinity Gel

HIS-Select® Nickel Affinity Gel (1:1 suspension in a 20% ethanol solution)

P6611

HIS-Select® Nickel Affinity Gel

HIS-Select® Cobalt Affinity Gel (1:1 suspension in a 30% ethanol solution)

H8162

HIS-Select® Cobalt Affinity Gel

PHOS-Select™ Iron Affinity Gel

P9740

PHOS-Select™ Iron Affinity Gel

capacity

15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)

capacity

>15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)

capacity

>15 mg/mL, agarose binding capacity (protein)(with an approx. 30 kDa protein)

capacity

≥2 μmol/mL, gel (phosphopeptide)(following a 30 minute incubation at 25°C)

technique(s)

affinity chromatography: suitable

technique(s)

protein purification: suitable

technique(s)

protein purification: suitable

technique(s)

-

form

suspension

form

(1:1 suspension in a 20% ethanol solution)

form

(1:1 suspension in a 30% ethanol solution)

form

suspension

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

HIS-Select High Flow Nickel Affinity Gel is an immobilized metal-ion affinity chromatography (IMAC) gel that is designed to specifically bind His-tag proteins (histidine containing proteins) in chromatographic systems with pressures up to 200 psi and a maximum linear flow rate of 3,000 cm/hr.

Application

Affinity gel is suitable for the purification of histidine tagged proteins (His-tag proteins) in native and denaturing conditions.
Nickel Affinity Gel for the purification of HIS TAG® proteins (histidine tagged proteins). HIS-Select® High Flow (HF) brings the superior selectivity of HIS-Select technology to a highly cross-linked agarose for higher flow rates and mechanical stability under pressure. HIS-Select HF is designed for production scale purification and FPLC™ applications. As with other HIS-Select products, the non-charged, hydrophilic linkage of the nickel-nitrilotriacetic acid (Ni-NTA) chelate group to the agarose ensures true one-step purification.

Features and Benefits

  • High selectivity for higher purity
  • Unique non-charged hydrophilic linkage reduced non-specific binding
  • Binding Capacity for histidine-tagged protein (His-tag protein) is greater than 15 mg/mL
  • Highly cross-linked agarose matrix allows for purification under higher pressures
  • Binding under denaturing or non-denaturing conditions
  • One-step purification

Physical form

1:1 suspension in a 30% ethanol solution

Preparation Note

Ethanol must be removed prior to use. Wash affinity gel in deionized water to remove the ethanol, then equilibrate with equilibration buffer that is provided.

Legal Information

FPLC is a trademark of Cytiva
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1A Inhalation - Flam. Liq. 3 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 - STOT SE 2

Target Organs

Eyes,Central nervous system, Respiratory Tract

WGK

WGK 3

Flash Point(F)

88.0 °F

Flash Point(C)

31.1 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

危险化学品
  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    SLCQ5051
    SLCL9242
    SLCG1131
    SLCB1729
    SLBR9492V

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    David S Pitcher et al.
    EBioMedicine, 2(7), 642-648 (2015-08-20)
    The proteasome inhibitor Bortezomib is used to treat multiple myeloma (MM). Bortezomib inhibits protein degradation by inactivating proteasomes' active-sites. MM cells are exquisitely sensitive to Bortezomib - exhibiting a low-nanomolar IC(50) - suggesting that minimal inhibition of degradation suffices to
    Minoru Tanaka et al.
    Nature chemical biology, 12(12), 1089-1096 (2016-10-25)
    Cellular signaling is often propagated by multivalent interactions. Multivalency creates avidity, allowing stable biophysical recognition. Multivalency is an attractive strategy for achieving potent binding to protein targets, as the affinity of bivalent ligands is often greater than the sum of
    Obtaining of Agr2 Specific Antibodies and Determination of the Agr2 Protein Distribution Pattern during Early Embryonic Development and Tadpole Regeneration in Xenopus laevis
    Ivanova, A.S. et al.
    Russian journal of developmental biology, 49, 393-397 (2018)
    Irving E Vega et al.
    Frontiers in neuroscience, 13, 845-845 (2019-08-29)
    The transition of tau proteins from its soluble physiological conformation to the pathological aggregate forms found in Alzheimer's disease and related dementias, is poorly understood. Therefore, understanding the process that modulates the formation of toxic tau oligomers and their conversion
    Kevin McCloskey et al.
    Journal of medicinal chemistry, 63(16), 8857-8866 (2020-06-12)
    DNA-encoded small molecule libraries (DELs) have enabled discovery of novel inhibitors for many distinct protein targets of therapeutic value. We demonstrate a new approach applying machine learning to DEL selection data by identifying active molecules from large libraries of commercial
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