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H7787

Millipore

HIS-Select® Spin Columns

Synonym(s):

His-Tag Spin Columns

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56
Product H7787 is not currently sold in your country. Contact Technical Service

shelf life

2 yr at 2‑8 °C

Quality Level

200

packaging

pkg of 10 ea
pkg of 50 ea

pore size

100 nm pore size

capacity

>0.5 mg/column

storage temp.

2-8°C

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88597H100918304
Hydrogen peroxide solution 30 % (w/w), for ultratrace analysis

16911

Hydrogen peroxide solution

Hydrogen peroxide solution 3%, suitable for microbiology

88597

Hydrogen peroxide solution

Hydrogen peroxide solution 30 % (w/w) in H2O, contains stabilizer

H1009

Hydrogen peroxide solution

Hydrogen peroxide solution 34.5-36.5%

18304

Hydrogen peroxide solution

grade

for inorganic trace analysis, for ultratrace analysis

grade

-

grade

-

grade

-

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

form

liquid

form

solution

form

liquid

form

-

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

2-8°C

anion traces

bromide (Br-): ≤10 μg/kg, fluoride (F-): ≤1000 μg/kg, phosphate (PO43-): ≤25 μg/kg, chloride (Cl-): ≤25 μg/kg, sulfate (SO42-): ≤50 μg/kg, nitrate (NO3-): ≤250 μg/kg

anion traces

-

anion traces

-

anion traces

-

General description

Kit contains 3 collection tubes for every spin column.

Application

Pre-packed and ready-to-use HIS-Select Spin Columns allow for fast and consistent purification of small-scale crude cell extracts containing histidine-tagged proteins. The spin columns contain a matrix of silica particles and uses the HIS-Select patented non-charged, hydrophilic nickel chelating linkage chemistry. HIS-Select Spin Columns are highly selective for histidine-tagged proteins and exhibit very low non-specific binding of other proteins. These convenient Spin Columns allow for fast, one-step purification for the isolation of at least 150 μg of histidine-tagged protein in less than 15 minutes.

Features and Benefits

  • High Selectivity for higher purity
  • Convenient and Ready-to-Use
  • Consistent protein binding each use
  • Compatible with CelLytic™ cell lysis reagents

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    0000439359
    0000439359
    0000437261
    0000437261
    SLCQ6970

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    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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    1. Which document(s) contains shelf-life or expiration date information for a given product?

      If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    2. How do I get lot-specific information or a Certificate of Analysis?

      The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    3. What is the binding capacity of the HIS-Select® Spin Column, Product H7787?

      The binding capacity of a HIS-Select Spin Column is >500 ug per column as determined witha ~30 kDa histidine tagged protein recovered under high levels of protein expression.

    4. Can HIS-Select® Spin Column, Product H7787, be re-used?

      We do not recommend re-using the columns. Each column is intended for one time use.

    5. How do I find price and availability?

      There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

    6. What is the Department of Transportation shipping information for this product?

      Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

    7. My question is not addressed here, how can I contact Technical Service for assistance?

      Ask a Scientist here.

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