GE29-0510-21
HisTrap™ High Performance
Cytiva 29-0510-21, pack of 1 mL
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shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 1 mL
manufacturer/tradename
Cytiva 29-0510-21
availability
not available in North America
parameter
flow rate
4 ml/min (1 ml), 5 ml/min (5ml) flow rate (H2O at room temperature)
42 psi (H2O at room temperature.)
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
7 mm
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning
2-14(Ni2+-stripped medium.)
working range
3-12(Ni2+-stripped medium)
capacity
≥40 mg binding capacity (histidine-tagged protein/ml medium)(H2O at room temperature.)
≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)
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General description
Application
Features and Benefits
- High binding capacity, at least 40 mg/ml chromatography medium.
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
- Negligible Ni2+ leakage
- Simple manual operation with a syringe, pump, or chromatography system such as AKTA design
Storage and Stability
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Warning
Hazard Statements
Precautionary Statements
Regulatory Information
Certificates of Analysis (COA)
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Articles
This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from Cytiva.
This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap™ FF columns and ÄKTAprime from Cytiva.
This page shows how to perform a purification of His-tagged membrane proteins.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
Protocols
This page shows how to condition membrane proteins for further analysis with products from Cytiva.
This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
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