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GE29-0486-84

HiTrap® Desalting

Cytiva 29-0486-84, pack of 5 mL

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UNSPSC Code:
23151816
NACRES:
NA.56

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 5 mL

manufacturer/tradename

Cytiva 29-0486-84

availability

not available in North America

storage condition

dry media

parameter

42 psi

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

matrix

cross-linked dextran

particle size

>20 μm (dry)

cleaning

2-13

working range

2-13

suitability

suitable for bioprocess medium

Related Categories

General description

HiTrap Desalting is a prepacked, ready to use column for group separation between high and low molecular weight substances, i.e., buffer exchange prior to or after different chromatographic steps, removal of low molecular weight contaminants or removal of reagents to terminate a reaction.

Application

Gel filtration has successfully been employed for size based separations of macromolecules since the late 1950s. Gel filtration, or size exclusion chromatography, may be performed in three principally different modes, depending on the size differences of the solutes to be separated (i) group separation (ii) fractionation (iii) determination of molecular mass (distributions) When the size difference is large, i.e. a factor of more than 10, we talk about group separation (e.g. desalting, buffer exchange) and when the size difference is small, i.e. a factor of 2 to 5, we talk about fractionation. The third mode is analytical gel filtration, e.g. determination of molecular mass distributions, where an array of molecular masses is to be separated. These different modes put different requirements on the equipment and media used and will yield widely different productivity in terms of information or material processed.

Features and Benefits

  • Handles sample volumes of 1.5 mL, or larger volumes by connecting up to five columns in series.
  • Replaces dialysis as the method of choice for desalting.
  • Desalting requires less than one minute due to the high rigidity of Sephadex G-25 and the optimized packing technology.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiTrap is a registered trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Regulatory Information

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