GE28-9893-36
HiLoad® 26/600 Superdex® 200 pg
Cytiva 28-9893-36, column L × I.D. 60 cm × 26 mm, 34 μm avg. part. size
Synonym(s):
HiLoad Superdex 200
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About This Item
Product Name
HiLoad® 26/600 Superdex® 200 pg, Cytiva 28-9893-36, column L × I.D. 60 cm × 26 mm, 34 μm avg. part. size
material
bed support (polyamide)
fiberglass end pieces (polypropylene reinforced)
glass column
form
prepacked column
crosslinking
cross-linked
mol wt
protein Mr 10-600 kDa (fractionation range)
Mr 1-100 kDa (dextrans fractionation range)
packaging
pkg of 1 ea
manufacturer/tradename
Cytiva 28-9893-36
storage condition
(20% Ethanol )
parameter
≤13 mL sample volume
0.3 Mpa
0.9-4.4 mL/min flow rate
technique(s)
liquid chromatography (LC): suitable
bed volume
320 mL
column L × I.D.
60 cm × 26 mm
matrix
cross-linked agarose, spherical
dextran-agarose particle platform
matrix active group
agarose
dextran-agarose phase
avg. part. size
34 μm
cleaning
1-14
working range
3-12
separation technique
size exclusion (SEC)
shipped in
ambient
storage temp.
2-30°C
Related Categories
Analysis Note
Application
Features and Benefits
- High resolution with short run times and good recovery.
- High capacity at high flow rates.
- Separations can be scaled up to production levels.
- Prepacked for convenience and reproducibility.
- 1/16” fittings for convenient connections to AKTA design systems.
General description
Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.
Preparation Note
Legal Information
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
Regulatory Information
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Articles
Understanding pressure drop in chromatography systems is crucial for optimal functionality across different parts of the system.
Protocols
Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.
Ensure chromatographic sample clarity to avoid column clogging, reduce washing needs, and prolong column life for purification.
Cytiva的Superdex是由葡聚糖和琼脂糖的复合基础基质组成的SEC介质。本页面介绍如何使用superdex执行分离任务。
Related Content
Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.
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