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GE28-9893-34

HiLoad^® 26/600 Superdex^® 75 pg

Name: HiLoad<SUP>®</SUP> 26/600 Superdex<SUP>®</SUP> 75 pg
Brand: SIGMA

Cytiva 28-9893-34, column L × I.D. 60 cm × 26 mm, 34 μm avg. part. size

Synonym(s):

Size exclusion chromatography

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About This Item

UNSPSC Code:

41115700

NACRES:

SB.52

material

glass column
glass fiber (polypropylene reinforced)

mol wt

3000-70000 Da (globular proteins)

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 28-9893-34

parameter

<13 mL sample volume
<2.6 mL/min flow rate
0.3 Mpa (42 psi)

bed volume

320 mL

column L × I.D.

60 cm × 26 mm

matrix

agarose dextran composite
dextran-agarose particle platform
polymeric

matrix active group

dextran-agarose phase
dextran-agarose phase

avg. part. size

34 μm

cleaning

1-14

working range

3-12

separation technique

size exclusion (SEC)

Related Categories

Cytiva™

General description

HiLoad^® 26/600 Superdex^® 75 prep grade are prepacked 53 mL XK columns designed for high-resolution preparative gel filtration chromatography separating small proteins and polynucleotides.

Superdex^® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex^® 30 pg), Mr 3 000 to 70 000 (Superdex^® 75 pg), and Mr 10 000 600 000 (Superdex^® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.

Features and Benefits

High resolution with short run times and good recovery.
High capacity at high flow rates.
Separations can be scaled up to production levels.
Prepacked for convenience and reproducibility.
1/16” fittings for convenient connections to AKTA design systems.

Suitability

All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile

for high-resolution preparative gel filtration chromatography separating proteins and polynucleotides

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Legal Information

HiLoad is a registered trademark of Cytiva

Superdex is a registered trademark of Cytiva

Pictograms

GHS02

Signal Word

Warning

Hazard Statements

H226

Precautionary Statements

P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Setting Pressure Limits for Size Exclusion Chromatography

Understanding pressure drop in chromatography systems is crucial for optimal functionality across different parts of the system.

Protocols

Performing a Separation with Superdex

Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.

Refolding of proteins using gel ﬁltration

This page shows how to refold proteins using gel filtration and products from Cytiva.

Sample Preparation for Size Exclusion Chromatography

Ensure chromatographic sample clarity to avoid column clogging, reduce washing needs, and prolong column life for purification.