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Merck
CN

GE28-9031-32

Protein A High Performance Spintrap™

Cytiva 28-9031-32, pack of 16 columns

Synonym(s):

High Performance Spintrap, Protein A Spintrap

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About This Item

NACRES:
NA.25
UNSPSC Code:
41106500

Key Documents

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Product Name

Protein A High Performance Spintrap™, Cytiva 28-9031-32, pack of 16 columns

ligand

native protein A

feature

wetted part: no

packaging

pack of 16 columns

manufacturer/tradename

Cytiva 28-9031-32

storage condition

(20% Ethanol)

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

cleaning

2-10

working range

3-9

capacity

>1 mg binding capacity(human IgG well)

storage temp.

2-8°C

Related Categories

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

Protein A Sepharose™ HP has high affinity for the Fc region of IgG from a variety of species. The two options in the Protein enrichment protocol allow the attached antigen (Protein of interest) to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.

There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.

Features and Benefits

  • For simple, small-scale antibody purification or enrichment of specific Proteins in prepacked, single-use spin columns that are ready to use.
  • Classic and cross-link protocols provide flexibility.
  • Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
  • Fast binding kinetics and high capacity provide high yield.
  • Prepacked with Protein A Sepharose™ High Performance for coupling of antibodies of IgG subclasses.
  • Easy scale-up with HiTrap® Protein A HP prepacked columns.

General description

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
protein A HP SpinTrap™ are designed for the enrichment of proteins of interest, immunoprecipitation and the purification of monoclonal and polyclonal antibodies from cell lysates and biological fluids. Contain protein A Sepharose™ HP.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Legal Information

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Regulatory Information

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Articles

Immunoprecipitation Techniques

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

Purification Using Protein A-based Chromatography Media

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

Protocols

Performing a Purification of IgG Antibodies with Protein A HP SpinTrap™ columns

This page shows how to separate IgG antibodies by affinity chromatography using Protein A HP SpinTrap columns from Cytiva.

Performing a Purification of IgG Antibodies with Protein G HP SpinTrap™/Ab SpinTrap™

This page shows how to separate IgG antibodies by affinity chromatography using Protein G HP SpinTrap™/Ab SpinTrap™ from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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