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manufacturer/tradename
Cytiva 25-0500-71
packaging
pkg of 50 preps
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capacity
100 μg binding capacity
greener alternative category
, Aligned
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General description
illustra™ RNAspin Mini Isolation Kit can be used to isolate total RNA from cultured cells, tissue, bacteria, or yeast. The isolated RNA is of a high enough quality and quantity (up to 100 μg) for multiple downstream experiments and sensitive enzymatic applications like RT-PCR, primer extension, and RNase protection assays. The RNAspin Mini Kit has been used for the isolation of high-quality total RNA from several types of source material including hard-to-lyse bacterial strains, and applied in downstream experiments such as quantitative reverse transcriptase PCR (QRT-PCR), Northern blot, and microarray experiments. The kit is supplied with a full protocol booklet with a detachable, quick reference protocol card, and all the necessary components including prefilters and DNase I.
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illustra™ RNAspin Mini Isolation Kit can be used to isolate total RNA from cultured cells, tissue, bacteria, or yeast. The isolated RNA is of a high enough quality and quantity (up to 100 μg) for multiple downstream experiments and sensitive enzymatic applications like RT-PCR, primer extension, and RNase protection assays. The RNAspin Mini Kit has been used for the isolation of high-quality total RNA from several types of source material including hard-to-lyse bacterial strains, and applied in downstream experiments such as quantitative reverse transcriptase PCR (QRT-PCR), Northern blot, and microarray experiments. The kit is supplied with a full protocol booklet with a detachable, quick reference protocol card, and all the necessary components including prefilters and DNase I.
Application
Features and Benefits
- High-quality output RNA from diverse sample types.
- Recovers high-quality total RNA due to the removal of genomic DNA through on-column DNase I treatment.
- For maximum yield and purity, prefilters are included to reduce lysate viscosity.
- Results can be obtained with even small amounts of precious sample.
- Lysis buffer is less susceptible to foaming to ensure valuable RNA sample is not wasted.
- Simple and convenient format that is suitable for all levels of expertise.
Analysis Note
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Certificates of Analysis (COA)
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Articles
This page shows possible causes and solutions for problems that may occur during RNA preparation.
This article describes the evaluation of Whatman FTA cards from Cytiva for their ability to collect, store, and isolate high-quality RNA from a variety of crude biological samples.
Protocols
Spectrophotometry can be used to estimate DNA or RNA concentration and to analyze the purity of the preparation.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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