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About This Item
Product Name
Ficoll®-Paque PREMIUM 1.073, liquid, sterile (endotoxins < 0.12 EU/mL), pack of 6 × 100 mL
sterility
sterile (endotoxins < 0.12 EU/mL)
form
liquid
shelf life
3 yr
packaging
pack of 6 × 100 mL
manufacturer/tradename
Cytiva 17-5446-52
storage condition
protect from light
dilution
(purification of lower density mononuclear cells from human peripheral blood)
color
Colorless to slightly yellow
pH range
5.5-7.5
density
1.073 g/mL
storage temp.
2-30°C
Related Categories
Analysis Note
Application
Features and Benefits
- Sterile, ready to use reagents used to prepare mononuclear cell preparations from peripheral blood, bone marrow, and umbilical cord blood by density gradient centrifugation.
- Manufactured under a Quality Management System certified to ISO 13485:2003.
- Low levels of endotoxin (< 0.12 EU/mL) secured and tested.
- Cytotoxicity grade 0.
- A Regulatory Support File (RSF) is available for Ficoll-Paque PREMIUM products.
General description
Preparation Note
Legal Information
Storage Class
12 - Non Combustible Liquids
Regulatory Information
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Articles
The effects of the mononuclear cell separation method described by Bøyum procedure are noted below, since research situations may arise in which they are significant.
Density Marker Beads facilitate Percoll gradient monitoring in biological separations.
This page shows methods to remove Percoll from a sample after centrifugation.
Protocols
To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.
The following procedure has been evaluated with Ficoll-Paque PLUS and is recommended for separation of normal blood samples for maximum reproducibility.
为了配置Percoll梯度液，必须先用生理盐水或细胞培养基将Percoll调至生理性渗透压。
以下操作步骤已接受Ficoll-Paque PLUS评估，推荐用于分离正常血样以尽可能提高可重现性。
Related Content
Data was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll for a particular cell or tissue type.
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