GE17-5446-02
Ficoll®-Paque PREMIUM 1.084
Cytiva 17-5446-02, pack of 6 × 100 mL
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form
liquid
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 6 × 100 mL
manufacturer/tradename
Cytiva 17-5446-02
color
Colorless to slightly yellow
pH range
5.5-7.5
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General description
Ficoll-Paque PREMIUM products are based on Ficoll-Paque PLUS (see Ficoll-Paque PLUS) which has been used for more than 30 yr as a sterile density medium for the isolation of high yields of mononuclear cells from bone marrow, peripheral blood, and cord blood. All Ficoll-Paque PREMIUM products differ from Ficoll-Paque PLUS in that they are manufactured under a Quality Management System certified to ISO 13485:2003.
Application
Can be used for isolating a broad range of human mononuclear cells including those of a higher density and for separating blood cells from mice or rats.
Can be used when isolating lower-density human mononuclear cells (e.g., mesenchymal stromal cells or monocytes).
Features and Benefits
- Sterile, ready to use reagents used to prepare mononuclear cell preparations from peripheral blood, bone marrow, and umbilical cord blood by density gradient centrifugation.
- Manufactured under a Quality Management System certified to ISO 13485:2003.
- Low levels of endotoxin (< 0.12 EU/mL) secured and tested.
- Cytotoxicity grade 0.
- A Regulatory Support File (RSF) is available for Ficoll-Paque PREMIUM products.
Storage and Stability
Analysis Note
Legal Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
This page shows methods to remove Percoll from a sample after centrifugation.
The effects of the mononuclear cell separation method described by Bøyum procedure are noted below, since research situations may arise in which they are significant.
Density Marker Beads facilitate Percoll gradient monitoring in biological separations.
The table was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll in various cell types.
Protocols
The following procedure has been evaluated with Ficoll-Paque PLUS and is recommended for separation of normal blood samples for maximum reproducibility.
To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.
Related Content
Data was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll for a particular cell or tissue type.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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