GE17-5282-02
GSTrap™ High Performance
Cytiva 17-5282-02, pack of 5 × 5 mL
Recommended Products
ligand
glutathione
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 17-5282-02
parameter
<20 mL/min flow rate
42 psi (H2O at room temperature.)
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning
3-12
working range
3-12
capacity
>7 mg/mL binding capacity (medium)(The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
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General description
GSTrap™ HP 1-ml and 5-ml columns are prepacked with Glutathione Sepharose™ High Performance medium, which provides high-resolution purifications, thus a more concentrated sample. GST-tagged proteins are purified from pretreated bacterial lysates by affinity chromatography using immobilized glutathione, such as Glutathione Sepharose™ High Performance. GST-tagged proteins are bound to the affinity medium, and impurities are removed by washing with binding buffer.
GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods.
Features and Benefits
- High resolution purification of GST-tagged proteins.
- Mild elution conditions preserving protein antigenicity and function.
- Fast, one-step purification.
- Easy to use with syringe, pump or a chromatography system.
Storage and Stability
Analysis Note
Other Notes
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Certificates of Analysis (COA)
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Articles
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.
This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.
This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.
Protocols
GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.
GSTrap™ affinity columns facilitate protein purification with Glutathione Sepharose® chromatography media for efficient purification processes.
Glutathione Sepharose® High Performance is recommended for high-resolution purification of GST-tagged proteins, providing sharp peaks and concentrated eluent.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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