GE17-0920-05
HiTrap® IMAC High Performance
Cytiva 17-0920-05, pack of 5 × 5 mL
Recommended Products
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 17-0920-05
parameter
42 psi (H2O at room temperature.)
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning
2-14(Medium uncharged with metal ions.)
working range
3-12
capacity
≥40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)
Related Categories
General description
Application
Features and Benefits
- For optimizing purification of histidine-tagged Proteins when Ni2+ is not the best choice of metal ion.
- Conveniently charge with your metal of choice.
- Small bead size for high-performance purification.
- High binding capacity.
Storage and Stability
Analysis Note
Other Notes
Legal Information
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
How to optimize purification of histidine-tagged proteins using Cytiva products.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products
Protocols
This page shows how to purify histidine-tagged proteins using HiTrap® IMAC HP and HiTrap® IMAC FF from Cytiva.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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