GE17-0727-20
SOURCE™ 15RPC
Cytiva 17-0727-20, pack of 10 mL
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ligand
matrix surface
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
feature
autoclavable 20 min at 121°C
packaging
pack of 10 mL
manufacturer/tradename
Cytiva 17-0727-20
matrix
polystyrene/divinylbenzene
avg. part. size
15 μm (monosized)
cleaning in place
1-14
working range
1-12
capacity
~10 mg binding capacity(~10 mg BSA /ml medium at 300 cm/h, ~30 mg bacitracin/ml medium at 300 cm/h, ~50 mg insulin/ml medium at 300 cm/h)
suitability
suitable for bioprocess medium
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General description
Application
High quality assurance standards in combination with the patented production method, particle structure is consistent both bead-to-bead and batch-to-batch.
A controlled pore size distribution and large specific surface area offer excellent capacity for a wide range of molecules, from small peptides and oligonucleotides up to large Proteins. With full pH range stability, SOURCE™ 15RPC is a strong alternative to silica-based RPC matrices.
SOURCE™ 15RPC is available in a range of different bulk pack sizes for easy scale-up and process development. As member of the BioProcess media range, SOURCE™ 15RPC meets industrial demands with security of supply and comprehensive technical and regulatory support.
Features and Benefits
- Mono-sized 15 μm rigid beads gives Low back pressures and high flow rates allowing for high productivity.
- Designed for high resolution reversed phase chromatography of Proteins, peptides and oligonucleotides.
- High chemical stability allowing for wide range of working conditions with good resistance to cleaning conditions at high pH
- BioProcess medium supported for industrial applications.
Storage and Stability
Analysis Note
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Certificates of Analysis (COA)
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Articles
“Ghosting” in chromatograms due to poor eluent quality; trace organic impurities concentrate, causing unknown peaks.
The different stages of working with SOURCE™ (Cytiva) for high-resolution separation are discussed, from purification to polishing and scaling up
This page discusses column packing and preparation techniques for reverse phase chromatography.
This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.
Protocols
In this section the practical aspect of Reverse Phased Chromatography ( RPC) is discussed including media and column selection and eluent selection and preparation.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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