GE17-0440-01
Con-A Sepharose™ 4B
Cytiva 17-0440-01, pack of 100 mL
Synonym(s):
Concanavalin A Sepharose
About This Item
ligand
concanavalin A (con A)
packaging
pack of 100 mL
manufacturer/tradename
Cytiva 17-0440-01
storage condition
(0.1 M acetate buffer pH 6, 1 M NaCl, 1 mM CaCl2, 1 mM MnCl2 and 1 mM MgCl, 20% Ethanol)
matrix
4% agarose
particle size
45-165 μm
average diameter
90 μm
cleaning in place
4-9
working range
4-6
storage temp.
2-8°C
Related Categories
General description
Con A Sepharose™ is Concanavalin A coupled to Sepharose™ 4B by the cyanogen bromide method. Concanavalin A (Con A) is a tetrameric metalloprotein isolated from Canavalia ensiformis (jack bean). Con A binds molcules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to Sepharose™ is routinely used for separation and purification of glycoproteins, polysaccharides and glycolipids.
Features and Benefits
- Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
- Group-specific adsorbent for molecules containing sugars.
- Highly suitable for isolation of cell surface glycoproteins from detergent-solubilized membranes
- Concanavalin A (Con A) binds molecules that contain α-D-mannose, α-D-glucose and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
- Highly suitable for isolation of cell surface glycoproteins from detergent-solubilized membranes
- Con A coupled to Sepharose™ 4B via cyanogen bromide activation
Storage and Stability
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Regulatory Information
Certificates of Analysis (COA)
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Articles
This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Protocols
This page shows how to purify or remove glycoproteins and polysaccharides with Con A Sepharose 4B, Lentil Lectin Sepharose 4B, Capto Lentil Lectin from Cytiva.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.
This page shows how to prepare samples for purification with affinity chromatography.
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