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GE11-0034-00

His Buffer Kit

Cytiva 11-0034-00

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UNSPSC Code:
41115700
NACRES:
NA.25

sterility

non-sterile

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 11-0034-00

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General description

His Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of histidine-tagged proteins according to recommended protocols. Moreover, method for optimization of imidazole concentration in the binding buffer is described.

Features and Benefits

His Buffer Kit can be used in combination with a number of column types, such as His SpinTrap, His MultiTrap FF, His MultiTrap HP, His GraviTrap, HisTrap FF, HisTrap FF crude and HisTrap HP

Storage and Stability

Store at 4 to 30 °C ()

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HisTrap is a trademark of Cytiva
MultiTrap is a trademark of Cytiva

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

How to optimize purification of histidine-tagged proteins using Cytiva products.

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

Protocols

This page describes a small-scale purification (minipreps) of histidine-tagged recombinant proteins using His SpinTrap and His SpinTrap Kit from Cytiva.

This page shows how to purify histidine-tagged recombinant proteins by gravity flow using His GraviTrap and His GraviTrap Kit from Cytiva.

This page shows magnetic bead-based purification and screening using His Mag Sepharose Ni from Cytiva.

Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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